气候变化领域集成服务门户(CCPortal): The life cycle of Dermacentor nuttalli from the Qinghai-Tibetan Plateau under laboratory conditions and detection of spotted fever group Rickettsia spp.

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DOI	10.3389/fvets.2023.1126266
	The life cycle of Dermacentor nuttalli from the Qinghai-Tibetan Plateau under laboratory conditions and detection of spotted fever group Rickettsia spp.
	Ma, Hejia; Ai, Jingkai; Kang, Ming; Li, Jixu; Sun, Yali
发表日期	2023
EISSN	2297-1769
卷号	10
英文摘要	Dermacentor nuttalli has been a focus of study because tick-borne pathogens have been widely identified in this tick from northern and southwestern China. The aim of this study was to characterize the life cycle of D. nuttalli under laboratory conditions and to detect spotted fever group (SFG) Rickettsia in the midgut and salivary glands of both field-collected and first laboratory generation adults. D. nuttalli ticks were collected in the field on the Qinghai-Tibetan Plateau from March to April 2021 and their life cycle was studied under laboratory conditions. Tick identify was molecularly confirmed, and SFG Rickettsia were detected in the midgut and salivary glands of males and females by PCR targeting different rickettsial genes. The results showed that the life cycle of D. nuttalli under laboratory conditions was completed in an average of 86.1 days. High positivity of Rickettsia spp. was detected in the midgut and salivary glands of both males (92.0%) and females (93.0%) of field-collected D. nuttalli ticks. However, a relatively lower positivity (4.0-6.0%) was detected in first laboratory generation adults. Furthermore, sequencing analysis showed that the Rickettsia sequences obtained in this study shared 98.6 to 100% nucleotide identity with Rickettsia slovaca and Rickettsia raoultii isolated from Dermacentor spp. in China. Phylogenetic analysis of Rickettsia spp. based on the gltA, ompA, ompB and sca4 genes revealed that the Rickettsia sequences obtained could be classified as belonging to R. slovaca and R. raoultii clades. This study described for the first time the life cycle of D. nuttalli from the Qinghai-Tibetan Plateau under laboratory conditions. Two species of SFG Rickettsia were detected in the midgut and salivary glands of males and females in both field-collected and first laboratory-generation adults of D. nuttalli. Our study provides new insights into pathogen detection in ticks in the Qinghai-Tibet Plateau, and the relationships among hosts, ticks, and pathogens.
关键词	Dermacentor nuttalli life cycle Rickettsia molecular identification Qinghai-Tibetan Plateau
英文关键词	ACARI IXODIDAE; HEBEI PROVINCE; TICKS; IDENTIFICATION; DIVERSITY; RAOULTII; BORRELIA; SPP.
WOS研究方向	Veterinary Sciences
WOS记录号	WOS:000950250700001
来源期刊	FRONTIERS IN VETERINARY SCIENCE
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283753
作者单位	Qinghai University; Qinghai University; Qinghai University
推荐引用方式 GB/T 7714	Ma, Hejia,Ai, Jingkai,Kang, Ming,et al. The life cycle of Dermacentor nuttalli from the Qinghai-Tibetan Plateau under laboratory conditions and detection of spotted fever group Rickettsia spp.[J],2023,10.
APA	Ma, Hejia,Ai, Jingkai,Kang, Ming,Li, Jixu,&Sun, Yali.(2023).The life cycle of Dermacentor nuttalli from the Qinghai-Tibetan Plateau under laboratory conditions and detection of spotted fever group Rickettsia spp..FRONTIERS IN VETERINARY SCIENCE,10.
MLA	Ma, Hejia,et al."The life cycle of Dermacentor nuttalli from the Qinghai-Tibetan Plateau under laboratory conditions and detection of spotted fever group Rickettsia spp.".FRONTIERS IN VETERINARY SCIENCE 10(2023).