气候变化领域集成服务门户(CCPortal): Transcriptome analysis of mRNAs, lncRNAs, and miRNAs in the skeletal muscle of Tibetan chickens at different developmental stages

CCPortal

DOI	10.3389/fphys.2023.1225349
	Transcriptome analysis of mRNAs, lncRNAs, and miRNAs in the skeletal muscle of Tibetan chickens at different developmental stages
	Li, Jie; Chen, Chuwen; Zhao, Ruipeng; Wu, Jinbo; Li, Zhixiong
发表日期	2023
EISSN	1664-042X
卷号	14
英文摘要	Introduction: As a valuable genetic resource, native birds can contribute to the sustainable development of animal production. Tibetan chickens, known for their special flavor, are one of the important local poultry breeds in the Qinghai-Tibet Plateau. However, Tibetan chickens have a slow growth rate and poor carcass traits compared with broilers. Although most of the research on Tibetan chickens focused on their hypoxic adaptation, there were fewer studies related to skeletal muscle development.Methods: Here, we performed the transcriptional sequencing of leg muscles from Tibetan chicken embryos at E (embryonic)10, E14, and E18.Results: In total, 1,600, 4,610, and 2,166 DE (differentially expressed) mRNAs, 210, 573, and 234 DE lncRNAs (long non-coding RNAs), and 52, 137, and 33 DE miRNAs (microRNAs) were detected between E10 and E14, E10 and E18, and E14 and E18, respectively. Functional prediction showed several DE mRNAs and the target mRNAs of DE lncRNAs and DE miRNAs were significantly enriched in sarcomere organization, actin cytoskeleton organization, myofibril, muscle fiber development, and other terms and pathways related to muscle growth and development. Finally, a lncRNA-miRNA-mRNA ceRNA (competing endogenous RNA) network associated with muscle growth and development, which contained 6 DE lncRNAs, 13 DE miRNAs, and 50 DE mRNAs, was constructed based on the screened DE RNAs by Gene Ontology (GO) enrichment. These DE RNAs may play a critical regulatory role in the skeletal muscle development of chickens.Discussion: The results provide a genomic resource for mRNAs, lncRNAs, and miRNAs potentially involved in the skeletal muscle development of chickens, which lay the foundation for further studies of the molecular mechanisms underlying skeletal muscle growth and development in Tibetan chickens.
关键词	chicken mRNA lncRNA miRNA skeletal muscle growth and development ceRNA
英文关键词	LONG NONCODING RNAS; ALIGNMENT; GENOME; BREAST
WOS研究方向	Physiology
WOS记录号	WOS:001044272000001
来源期刊	FRONTIERS IN PHYSIOLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283725
作者单位	Southwest Minzu University; Southwest Minzu University; Southwest Minzu University
推荐引用方式 GB/T 7714	Li, Jie,Chen, Chuwen,Zhao, Ruipeng,et al. Transcriptome analysis of mRNAs, lncRNAs, and miRNAs in the skeletal muscle of Tibetan chickens at different developmental stages[J],2023,14.
APA	Li, Jie,Chen, Chuwen,Zhao, Ruipeng,Wu, Jinbo,&Li, Zhixiong.(2023).Transcriptome analysis of mRNAs, lncRNAs, and miRNAs in the skeletal muscle of Tibetan chickens at different developmental stages.FRONTIERS IN PHYSIOLOGY,14.
MLA	Li, Jie,et al."Transcriptome analysis of mRNAs, lncRNAs, and miRNAs in the skeletal muscle of Tibetan chickens at different developmental stages".FRONTIERS IN PHYSIOLOGY 14(2023).