气候变化领域集成服务门户(CCPortal): Establishment of acute liver failure model in Tibetan miniature pig and verified by dual plasma molecular adsorption system

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DOI	10.1177/03913988221145501
	Establishment of acute liver failure model in Tibetan miniature pig and verified by dual plasma molecular adsorption system
	Wang, Yi; Zhou, Chenjie; Fu, Yu; Zhang, Linya; Liu, Shusong; Cai, Lei; Jiang, Zesheng; Xu, Xiaoping; Feng, Lei; Gao, Yi
发表日期	2023
ISSN	0391-3988
EISSN	1724-6040
起始页码	141
结束页码	152
卷号	46 期号:3
英文摘要	Background: Acute liver failure (ALF) is a severe liver disease with high morbidity and mortality rates. Animal models are important for research on ALF. This study aimed to establish a reproducible, Tibetan miniature pig model of D-galactosamine-induced ALF and verify it using a dual plasma molecular adsorption system (DPMAS). Methods: Tibet miniature pigs were randomly divided into four groups (A, B, C, D) after catheterization. D-galactosamine (D-gal) at 0.45, 0.40, 0.35, and 0.35 g/kg body weight, respectively, was injected through the catheter. Group D was treated with DPMAS 48 h after D-gal administration. Vital signs and blood index values were recorded every 12 h after D-gal administration. H&E, TUNEL, Ki67, and Masson staining tests were performed. Results: After D-gal administration, Tibetan miniature pigs developed different degrees of debilitation, loss of appetite, and jaundice. Survival times of groups A, B, C, and D were 39.7 +/- 5.9, 53.0 +/- 12.5,61.3 +/- 8.1, and 61 +/- 7 h, respectively. Blood levels of ALT, AST, TBIL, ammonia, PT, and inflammation factors significantly increased compared with baseline levels in the different groups (Ps < 0.05). Pathological results revealed a clear liver cell necrosis positive correlation with D-gal dose. However, DPMAS did not increase the survival time in ALF, ammonia, or liver cell necrosis. Conclusion: We successfully established a reproducible Tibetan miniature pig model of d-galactosamine-induced ALF, and we believe that a dosage of 0.35 g/kg is optimal.
关键词	Acute liver failure Tibetan miniature pig d-galactosamine DPMAS animal model
英文关键词	FULMINANT HEPATIC-FAILURE; RESERVOIR BIOARTIFICIAL LIVER; ANIMAL-MODELS; SURVIVAL; EXCHANGE; DEVICE; VIRUS; TRIAL
WOS研究方向	Engineering, Biomedical ; Transplantation
WOS记录号	WOS:000908098200001
来源期刊	INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283711
作者单位	Southern Medical University - China; Southern Medical University - China; Southern Medical University - China
推荐引用方式 GB/T 7714	Wang, Yi,Zhou, Chenjie,Fu, Yu,et al. Establishment of acute liver failure model in Tibetan miniature pig and verified by dual plasma molecular adsorption system[J],2023,46(3).
APA	Wang, Yi.,Zhou, Chenjie.,Fu, Yu.,Zhang, Linya.,Liu, Shusong.,...&Gao, Yi.(2023).Establishment of acute liver failure model in Tibetan miniature pig and verified by dual plasma molecular adsorption system.INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS,46(3).
MLA	Wang, Yi,et al."Establishment of acute liver failure model in Tibetan miniature pig and verified by dual plasma molecular adsorption system".INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS 46.3(2023).