气候变化领域集成服务门户(CCPortal): Regulation mechanism and functional verification of key functional genes regulating muscle development in black Tibetan sheep

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DOI	10.1016/j.gene.2023.147375
	Regulation mechanism and functional verification of key functional genes regulating muscle development in black Tibetan sheep
	Wu, Zhanyue; Liu, Meng; Yan, Mingyi; Dong, Shutong; Wu, Sen
发表日期	2023
ISSN	0378-1119
EISSN	1879-0038
卷号	868
英文摘要	Black Tibetan sheep is a branch of Tibetan sheep on the Qinghai-Tibet Plateau (QTP). It is mainly distributed in Guinan County, Qinghai Province. In order to accurately identify the core regulatory genes in the process of muscle development of black Tibetan sheep, further explore the physiological processes of growth, development and myogenesis of black Tibetan sheep, and carry out molecular breeding of black Tibetan sheep, this experiment took the unique black Tibetan sheep on the Qinghai-Tibet Plateau as the experimental object, and selected three stages of 4-month-old embryo (embryonic stage, MF group), 10-month-old(breeding stage, ML group) and 36month-old (adult, MA group). The longissimus dorsi tissues of 3 sheep were taken at each stage to quantify the expression of genes during muscle development at different developmental stages. Meanwhile, overexpression and interference techniques were used to detect the role of core genes in the proliferation of primary muscle cells of black Tibetan sheep. In the process from embryonic stage to mature stage and adulthood, more than 1000 genes were up-regulated and more than 4000 down-regulated in black Tibetan sheep, while from breeding to adulthood, there were only 51 up-regulated genes and 83 down-regulated genes. About 998 genes were newly identified in each group. During muscle development from embryonic stage to mature stage to adulthood, two significant differential trend gene sets of Profile1 and Profile 6 were screened and identified, in which there were 121 and 31 core regulatory genes identified, respectively. In the trend of first decreasing and then stable expression in the whole development stage, 121 genes are core regulatory transcripts, which are mainly related to axonal guidance, cell cycle and other functions. 31 genes are core regulatory transcripts in the first rising and then stable expression, which are mainly related to biological metabolic pathway, oxidative phosphorylation and other processes. In the MF-ML stage, 75 genes were selected as the core regulatory gene set, the core genes were PTEN, AKT3, etc., and there were 134 differentially expressed genes in the ML-MA stage, and the core regulatory genes were IL6, ABCA1 and so on. In the MF-ML stage, the core gene set widely plays a role in cell components, cell matrix and other biological processes, while in the ML-MA stage, the core gene set widely plays a role in cell migration, cell differentiation, tissue development and so on. Adenovirus vector overexpressed and interfered with the core gene PTEN in primary muscle satellite cells of black Tibetan sheep shown that, interference and overexpression of PTEN would correspondingly increase and decrease the expression of other core genes, like AKT3, CKD2, CCNB1, ERBB3, HDAC2, but the specific interaction mechanism of each gene still needs to be further explored.
关键词	Black Tibetan sheep Transcriptome RNA-seq Gene expression
英文关键词	MEAT QUALITY; RNA-SEQ; TRANSCRIPTOME; AGE
WOS研究方向	Genetics & Heredity
WOS记录号	WOS:000958630100001
来源期刊	GENE
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283677
作者单位	Qinghai University; Ministry of Agriculture & Rural Affairs
推荐引用方式 GB/T 7714	Wu, Zhanyue,Liu, Meng,Yan, Mingyi,et al. Regulation mechanism and functional verification of key functional genes regulating muscle development in black Tibetan sheep[J],2023,868.
APA	Wu, Zhanyue,Liu, Meng,Yan, Mingyi,Dong, Shutong,&Wu, Sen.(2023).Regulation mechanism and functional verification of key functional genes regulating muscle development in black Tibetan sheep.GENE,868.
MLA	Wu, Zhanyue,et al."Regulation mechanism and functional verification of key functional genes regulating muscle development in black Tibetan sheep".GENE 868(2023).