气候变化领域集成服务门户(CCPortal): Chryseobacterium herbae Isolated from the Rhizospheric Soil of Pyrola calliantha H. Andres in Segrila Mountain on the Tibetan Plateau

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DOI	10.3390/microorganisms11082017
	Chryseobacterium herbae Isolated from the Rhizospheric Soil of Pyrola calliantha H. Andres in Segrila Mountain on the Tibetan Plateau
	Zhang, Li; Wang, Yan; Kong, Delong; Ma, Qingyun; Li, Yan; Xing, Zhen; Ruan, Zhiyong
发表日期	2023
EISSN	2076-2607
卷号	11 期号:8
英文摘要	A non-motile, Gram-staining-negative, orange-pigmented bacterium called herbae pc1-10(T) was discovered in Tibet in the soil around Pyrola calliantha H. Andres' roots. The isolate thrived in the temperature range of 10-30 degrees C (optimal, 25 degrees C), pH range of 5.0-9.0 (optimum, pH = 6.0), and the NaCl concentration range of 0-1.8% (optimal, 0%). The DNA G+C content of the novel strain was 37.94 mol%. It showed the function of dissolving organophosphorus, acquiring iron from the environment by siderophore and producing indole acetic acid. Moreover, the genome of strain herbae pc1-10(T) harbors two antibiotic resistance genes (IND-4 and AdeF) encoding a beta-lactamase, and the membrane fusion protein of the multidrug efflux complex AdeFGH; antibiotic-resistance-related proteins were detected using the Shotgun proteomics technology. The OrthoANIu values between strains Chryseobacterium herbae pc1-10(T); Chryseobacterium oleae CT348T; Chryseobacterium kwangjuense KJ1R5(T); and Chryseobacterium vrystaatense R-23566(T) were 90.94%, 82.96%, and 85.19%, respectively. The in silico DDH values between strains herbae pc1-10(T); C. oleae CT348(T); C. kwangjuense KJ1R5(T); and C. vrystaatense R-23566(T) were 41.7%, 26.6%, and 29.7%, respectively. Chryseobacterium oleae, Chryseobacterium vrystaatense, and Chryseobacterium kwangjuense, which had 16S rRNA gene sequence similarity scores of 97.80%, 97.52%, and 96.75%, respectively, were its closest phylogenetic relatives. Chryseobacterium herbae sp. nov. is proposed as the designation for the strain herbae pc1-10(T) (=GDMCC 1.3255 = JCM 35711), which represented a type species based on genotypic and morphological characteristics. This study provides deep knowledge of a Chryseobacterium herbae characteristic description and urges the need for further genomic studies on microorganisms living in alpine ecosystems, especially around medicinal plants.
关键词	Chryseobacterium herbae sp. nov.alpine ecosystems rhizosphere soil Gram-staining-negative Pyrola calliantha H. Andres genomic shotgun proteomics
英文关键词	GROWTH-PROMOTING BACTERIUM; SP NOV.; EMENDED DESCRIPTIONS; DAECHEONGENSE; SEQUENCE; DEFLUVII; CHICKEN; GLEUM; WATER; ACID
WOS研究方向	Microbiology
WOS记录号	WOS:001055890800001
来源期刊	MICROORGANISMS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283659
作者单位	Yantai University; Chinese Academy of Agricultural Sciences; Institute of Agricultural Resources & Regional Planning, CAAS; Tibet Agriculture & Animal Husbandry University; Huazhong Agricultural University
推荐引用方式 GB/T 7714	Zhang, Li,Wang, Yan,Kong, Delong,et al. Chryseobacterium herbae Isolated from the Rhizospheric Soil of Pyrola calliantha H. Andres in Segrila Mountain on the Tibetan Plateau[J],2023,11(8).
APA	Zhang, Li.,Wang, Yan.,Kong, Delong.,Ma, Qingyun.,Li, Yan.,...&Ruan, Zhiyong.(2023).Chryseobacterium herbae Isolated from the Rhizospheric Soil of Pyrola calliantha H. Andres in Segrila Mountain on the Tibetan Plateau.MICROORGANISMS,11(8).
MLA	Zhang, Li,et al."Chryseobacterium herbae Isolated from the Rhizospheric Soil of Pyrola calliantha H. Andres in Segrila Mountain on the Tibetan Plateau".MICROORGANISMS 11.8(2023).