气候变化领域集成服务门户(CCPortal): Establishment of Tibetan-Sheep-Specific SNP Genetic Markers

CCPortal

DOI	10.3390/agriculture13020322
	Establishment of Tibetan-Sheep-Specific SNP Genetic Markers
	Liang, Benmeng; Zhao, Yuhetian; Pu, Yabin; He, Xiaohong; Han, Jiangang; Danzeng, Baima; Ma, Yuehui; Liu, Jianfeng; Jiang, Lin
发表日期	2023
EISSN	2077-0472
卷号	13 期号:2
英文摘要	Tibetan sheep are one of the three major coarse sheep breeds in China, and they possess a long history of formation. However, few studies have been conducted on the identification of Tibetan sheep breeds at the molecular level. In this study, a total of 448 individuals from 24 Tibetan sheep populations in the 5 regions of Tibet, Qinghai, Gansu, Yunnan, and Sichuan were analyzed using the Affymetrix Ovine SNP 600K high-density chip to construct specific single-nucleoside polymorphism (SNP) genetic marker sets of Tibetan sheep breeds. Firstly, the genetic structure analysis showed that Yunnan-Tibetan sheep (NL, Ninglang; JC, Jianchuan), Zuogong (ZG), Heizang (HZ), Gongga (GG,) and Tao sheep (TS) can be clearly distinguished from other Tibetan sheep populations. Next, based on the population differentiation index FST, the PCA and NJ tree results showed that only 60 specific SNPs can successfully separate Tibetan sheep in the Yunnan region from Tibetan sheep in other regions, and the distinguishing effect on Yunnan-Tibetan sheep reached 100%. Using the same method, we found that 4 Tibetan sheep breeds, including Zuogong (ZG, 20 SNPs), Heizang (HZ, 60 SNPs), Gongga (GG, 60 SNPs), and Tao sheep (TS, 30 SNPs), can also be distinguished from other Tibetan sheep populations with only a few SNP loci (20-60), and the distinguishing effect reached 100%. Overall, we successfully obtained a Yunnan region-specific (60 SNPs) genetic marker set and 4 breed-specific SNP genetic marker sets (20-60 SNPs) for the first time for the identification of Tibetan sheep breeds at the molecular level. These made up for the lack of genetic marker sets for the identification of Tibetan sheep breeds, and provided a genomic basis for the scientific classification and accurate identification of livestock and poultry genetic resources on the Qinghai-Tibet Plateau.
关键词	Tibetan sheep 600K high-density chips population differentiation index FST genetic marker sets
英文关键词	POPULATION-STRUCTURE; SELECTION; IDENTIFICATION; POLYMORPHISMS; ADAPTATIONS; DIVERSITY; REVEALS
WOS研究方向	Agronomy
WOS记录号	WOS:000938594700001
来源期刊	AGRICULTURE-BASEL
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283624
作者单位	Chinese Academy of Agricultural Sciences; China Agricultural University; China Agricultural University; Ministry of Agriculture & Rural Affairs; University College Dublin
推荐引用方式 GB/T 7714	Liang, Benmeng,Zhao, Yuhetian,Pu, Yabin,et al. Establishment of Tibetan-Sheep-Specific SNP Genetic Markers[J],2023,13(2).
APA	Liang, Benmeng.,Zhao, Yuhetian.,Pu, Yabin.,He, Xiaohong.,Han, Jiangang.,...&Jiang, Lin.(2023).Establishment of Tibetan-Sheep-Specific SNP Genetic Markers.AGRICULTURE-BASEL,13(2).
MLA	Liang, Benmeng,et al."Establishment of Tibetan-Sheep-Specific SNP Genetic Markers".AGRICULTURE-BASEL 13.2(2023).