气候变化领域集成服务门户(CCPortal): Update on the genetic diversity and population structure of Echinococcus granulosus in Gansu Province, Tibet Autonomous Region, and Xinjiang Uygur Autonomous Region, Western China, inferred from mitochondrial cox1, nad1, and nad5 sequences

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DOI	10.1007/s00436-023-07811-9
	Update on the genetic diversity and population structure of Echinococcus granulosus in Gansu Province, Tibet Autonomous Region, and Xinjiang Uygur Autonomous Region, Western China, inferred from mitochondrial cox1, nad1, and nad5 sequences
	Shumuye, Nigus Abebe; Li, Li; Ohiolei, John Asekhaen; Qurishi, Sayed Ajmal; Li, Wen-Hui; Zhang, Nian-Zhang; Wu, Yan-Tao; Wu, Yao-Dong; Gao, Sheng-Zhi; Zhang, Fu-Heng; Tian, Xue-Qi; Tian, Wen-Jun; Fu, Yong; Wang, Xie-Zhong; Pan, Yong-Hong; Zhan, Fang; Zhang, Lin-Sheng; Guo, Ming-Kuan; Li, Wen-Dong; Fu, Bao-Quan; Yan, Hong-Bin; Jia, Wan-Zhong
发表日期	2023
ISSN	0932-0113
EISSN	1432-1955
起始页码	1107
结束页码	1126
卷号	122 期号:5
英文摘要	The identification of additional Echinococcus granulosus sensu lato (s.l.) complex species/genotypes in recent years raises the possibility that there might be more variation among this species in China than is currently understood. The aim of this study was to explore intra- and inter-species variation and population structure of Echinococcus species isolated from sheep in three areas of Western China. Of the isolates, 317, 322, and 326 were successfully amplified and sequenced for cox1, nad1, and nad5 genes, respectively. BLAST analysis revealed that the majority of the isolates were E. granulosus s.s., and using the cox1, nad1, and nad5 genes, respectively, 17, 14, and 11 isolates corresponded to Elodea canadensis (genotype G6/G7). In the three study areas, G1 genotypes were the most prevalent. There were 233 mutation sites along with 129 parsimony informative sites. A transition/transversion ratio of 7.5, 8, and 3.25, respectively, for cox1, nad1, and nad5 genes was obtained. Every mitochondrial gene had intraspecific variations, which were represented in a star-like network with a major haplotype with observable mutations from other distant and minor haplotypes. The Tajima's D value was significantly negative in all populations, indicating a substantial divergence from neutrality and supporting the demographic expansion of E. granulosus s.s. in the study areas. The phylogeny inferred by the maximum likelihood (ML) method using nucleotide sequences of cox1-nad1-nad5 further confirmed their identity. The nodes assigned to the G1, G3, and G6 clades as well as the reference sequences utilized had maximal posterior probability values (1.00). In conclusion, our study confirms the existence of a significant major haplotype of E. granulosus s.s. where G1 is the predominant genotype causing of CE in both livestock and humans in China.
关键词	Echinococcus granulosus Genetic diversity China Sheep Mitochondrial gene Population structure
英文关键词	CYSTIC ECHINOCOCCOSIS; SENSU-STRICTO; DNA-SEQUENCE; GENOTYPE G1; MOLECULAR EPIDEMIOLOGY; INTERMEDIATE HOSTS; HUMAN HYDATIDOSIS; CERVID STRAIN; VARIABILITY; QINGHAI
WOS研究方向	Parasitology
WOS记录号	WOS:000952258800002
来源期刊	PARASITOLOGY RESEARCH
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283595
作者单位	Lanzhou University; Chinese Academy of Agricultural Sciences; Lanzhou Veterinary Research Institute, CAAS; Mekelle University; Qinghai University
推荐引用方式 GB/T 7714	Shumuye, Nigus Abebe,Li, Li,Ohiolei, John Asekhaen,et al. Update on the genetic diversity and population structure of Echinococcus granulosus in Gansu Province, Tibet Autonomous Region, and Xinjiang Uygur Autonomous Region, Western China, inferred from mitochondrial cox1, nad1, and nad5 sequences[J],2023,122(5).
APA	Shumuye, Nigus Abebe.,Li, Li.,Ohiolei, John Asekhaen.,Qurishi, Sayed Ajmal.,Li, Wen-Hui.,...&Jia, Wan-Zhong.(2023).Update on the genetic diversity and population structure of Echinococcus granulosus in Gansu Province, Tibet Autonomous Region, and Xinjiang Uygur Autonomous Region, Western China, inferred from mitochondrial cox1, nad1, and nad5 sequences.PARASITOLOGY RESEARCH,122(5).
MLA	Shumuye, Nigus Abebe,et al."Update on the genetic diversity and population structure of Echinococcus granulosus in Gansu Province, Tibet Autonomous Region, and Xinjiang Uygur Autonomous Region, Western China, inferred from mitochondrial cox1, nad1, and nad5 sequences".PARASITOLOGY RESEARCH 122.5(2023).