气候变化领域集成服务门户(CCPortal): Tyrosine phosphorylation of band 3 impairs the storage quality of suspended red blood cells in the Tibetan high-altitude polycythemia population

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DOI	10.1186/s12967-023-04428-5
	Tyrosine phosphorylation of band 3 impairs the storage quality of suspended red blood cells in the Tibetan high-altitude polycythemia population
	Wu, Xiaodong; Liu, Zhijuan; Hao, Doudou; Zhao, Qin; Li, Wanjing; Xie, Maodi; Feng, Xia; Liao, Xia; Chen, Siyuan; Wang, Siyu; Zhou, Chaohua; Long, Wenchun; Zhong, Yajun; Li, Shen; Cao, Ye; Wang, Hong; Wang, Aiping; Xu, Yuehong; Huang, Min; Liu, Jiaxin; Zhong, Rui; Wu, Yunhong; He, Zeng
发表日期	2023
EISSN	1479-5876
卷号	21 期号:1
英文摘要	Due to environmental hypoxia on the Tibetan Plateau, local residents often exhibit a compensative increase in hemoglobin concentration to maintain the body's oxygen supply. However, increases in hemoglobin and hematocrit (Hct) pose a serious challenge to the quality of stored suspended red blood cells (SRBCs) prepared from the blood of high-hemoglobin populations, especially populations at high altitude with polycythemia in Tibet. To explore the difference in storage quality of SRBCs prepared from plateau residents with a high hemoglobin concentration, blood donors were recruited from Tibet (> 3600 m) and Chengdu (approximate to 500 m) and divided into a high-altitude control (HAC) group, high-altitude polycythemia (HAPC) group and lowland control (LLC) group according to their hemoglobin concentration and altitude of residence. The extracellular acidification rate (ECAR), pyruvate kinase (PK) activity and band 3 tyrosine phosphorylation were analyzed on the day of blood collection. Then, whole-blood samples were processed into SRBCs, and storage quality parameters were analyzed aseptically on days 1, 14, 21 and 35 of storage. Overall, we found that tyrosine 21 phosphorylation activated glycolysis by releasing glycolytic enzymes from the cytosolic domain of band 3, thus increasing glucose consumption and lactate accumulation during storage, in the HAPC group. In addition, band 3 tyrosine phosphorylation impaired erythrocyte deformability, accompanied by the highest hemolysis rate in the HAPC group, during storage. We believe that these results will stimulate new ideas to further optimize current additive solutions for the high-hemoglobin population in Tibet and reveal new therapeutic targets for the treatment of HAPC populations.
英文关键词	TYR-PHOSPHORYLATION; HUMAN ERYTHROCYTES; MEMBRANE; PROTEIN
WOS研究方向	Medicine, Research & Experimental
WOS记录号	WOS:001080544900006
来源期刊	JOURNAL OF TRANSLATIONAL MEDICINE
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283553
作者单位	Chinese Academy of Medical Sciences - Peking Union Medical College; Institute of Blood Transfusion - CAMS; Peking Union Medical College; Sichuan University
推荐引用方式 GB/T 7714	Wu, Xiaodong,Liu, Zhijuan,Hao, Doudou,et al. Tyrosine phosphorylation of band 3 impairs the storage quality of suspended red blood cells in the Tibetan high-altitude polycythemia population[J],2023,21(1).
APA	Wu, Xiaodong.,Liu, Zhijuan.,Hao, Doudou.,Zhao, Qin.,Li, Wanjing.,...&He, Zeng.(2023).Tyrosine phosphorylation of band 3 impairs the storage quality of suspended red blood cells in the Tibetan high-altitude polycythemia population.JOURNAL OF TRANSLATIONAL MEDICINE,21(1).
MLA	Wu, Xiaodong,et al."Tyrosine phosphorylation of band 3 impairs the storage quality of suspended red blood cells in the Tibetan high-altitude polycythemia population".JOURNAL OF TRANSLATIONAL MEDICINE 21.1(2023).