气候变化领域集成服务门户(CCPortal): Land use change alters phosphatase enzyme activity and phosphatase-harboring microbial abundance in the subalpine ecosystem of southeastern Qinghai-Tibet Plateau, China

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DOI	10.1016/j.ecolind.2023.110416
	Land use change alters phosphatase enzyme activity and phosphatase-harboring microbial abundance in the subalpine ecosystem of southeastern Qinghai-Tibet Plateau, China
	Azene, Belayneh; Zhu, Renhuan; Pan, Kaiwen; Sun, Xiaoming; Nigussie, Yalemzewd; Gruba, Piotr; Raza, Ali; Guadie, Awoke; Wu, Xiaogang; Zhang, Lin
发表日期	2023
ISSN	1470-160X
EISSN	1872-7034
卷号	153
英文摘要	In the presence of limited phosphorus (P) in terrestrial ecosystems, exploring how land use change (LUC) affects phosphatase enzyme activity and microbial communities is important for managing soil P, because microorganisms carry out the majority of P cycling in the soil through producing phosphatase enzymes. In this study, we explored the impact of LUC on P availability, phosphatase enzyme, the abundance of phosphatase-encoding genes, and microorganisms. We collected 168 soil samples at soil depths of 0-20 cm and 20-40 cm from seven sampling sites, each of which represented by four different land uses: artificial forests (AF), farmlands (FL), natural forests (NF), and shrubland (SL). We analyzed phosphatase-encoding genes and microbes from metagenome datasets. Results indicated that P availability substantially increased following NF to FL conversion. In contrast, phosphatase enzyme activity significantly decreases when NF is converted to different land uses, due to the decline of soil organic carbon (SOC), moisture content (MC) and total nitrogen (TN). We have also detected 13 P solubilizing and mineralizing genes. The phoD and gcd were the dominant mineralizing and solubilizing genes, respectively. Farmland had higher gcd gene abundance, while NF had significantly higher phoD gene abundance. The gcd gene abundance were mainly governed by pH and total P, whereas pH and available P were the primary factors controlling phoD gene. MC, SOC and TN regulated other genes detected in this study. With regard to the dominant gcd-harboring phyla, Acidobacteria, Proteobacteria, Bacteroidetes and Gemmatimonadetes were the dominant, while Proteobacteria, Acidobacteria, Actinobacteria, and Candidatus_Rokubacteria were the dominant phoD-harboring phyla. The majority of gcd and phoD-harboring microorganisms were primarily controlled by pH, available P and total P. However, some phyla also regulated by MC, SOC, and TN. In general, our findings suggested that LUC significantly alters phosphatase enzymes and the abundance of phosphate-encoding genes and microbes. These changes have significant implications for soil P cycling.
关键词	gcd -Encoding gene gcd -Harboring microbes Land use change phoD -Encoding gene phoD -Harboring microbes Phosphatase enzymes
英文关键词	LONG-TERM FERTILIZATION; SOIL-MICROORGANISMS; BACTERIAL COMMUNITY; CLIMATE-CHANGE; FOREST SOILS; PHOSPHORUS; TERRESTRIAL; DIVERSITY; RESPONSES; DYNAMICS
WOS研究方向	Biodiversity Conservation ; Environmental Sciences
WOS记录号	WOS:001012644100001
来源期刊	ECOLOGICAL INDICATORS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283531
作者单位	Chinese Academy of Sciences; Chengdu Institute of Biology, CAS; Chinese Academy of Sciences; Chengdu Institute of Biology, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Agricultural University Krakow; Arba Minch University
推荐引用方式 GB/T 7714	Azene, Belayneh,Zhu, Renhuan,Pan, Kaiwen,et al. Land use change alters phosphatase enzyme activity and phosphatase-harboring microbial abundance in the subalpine ecosystem of southeastern Qinghai-Tibet Plateau, China[J],2023,153.
APA	Azene, Belayneh.,Zhu, Renhuan.,Pan, Kaiwen.,Sun, Xiaoming.,Nigussie, Yalemzewd.,...&Zhang, Lin.(2023).Land use change alters phosphatase enzyme activity and phosphatase-harboring microbial abundance in the subalpine ecosystem of southeastern Qinghai-Tibet Plateau, China.ECOLOGICAL INDICATORS,153.
MLA	Azene, Belayneh,et al."Land use change alters phosphatase enzyme activity and phosphatase-harboring microbial abundance in the subalpine ecosystem of southeastern Qinghai-Tibet Plateau, China".ECOLOGICAL INDICATORS 153(2023).