气候变化领域集成服务门户(CCPortal): Structural characterization of a low-molecular weight linear O-acetyl-glucomannan in Lilium lancifolium from Tibet and its protected H2O2-induced oxidative stress in HUVEC cells

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DOI	10.1186/s40538-024-00539-3
	Structural characterization of a low-molecular weight linear O-acetyl-glucomannan in Lilium lancifolium from Tibet and its protected H2O2-induced oxidative stress in HUVEC cells
	Yuan, Lei; Zhong, ZhengChang; Liu, Yu; Quan, Hong; Lan, XiaoZhong
发表日期	2024
EISSN	2196-5641
卷号	11 期号:1
英文摘要	In recent years, the biological activity of plant polysaccharides has received increasing attention. Polysaccharides, as one of the main components of lilies, have pharmacological effects in regulating immunity, anti-tumor, antioxidant, antibacterial, and hypoglycemic effects. To systematically analyze the structural characteristics of the polysaccharide, the polysaccharide LP-1 was prepared from Lilium lancifolium bulbs by water extraction and ethanol precipitation, ion exchange chromatography and gel filtration chromatography. Structural characterizations show that the weight-average relative molecular weight of LP-1 is 5.3 kDa. LP-1 consists of mannose and glucose at a molar ratio of 1.4:1. Its primary structure is Glcp-alpha-D-(1 -> {4)-beta-D-Glcp-(1 -> 4)-beta-D-2-O-acetyl-Manp-(1 -> [4)-beta-D-Glcp-(1 -> 4)-beta-D-Glcp-(1 -> 4)-beta-D-Manp-(1 -> 4)-beta-D-Manp-(1 -> 4)-beta-D-Manp-(1 -> 4)-beta-D-Manp-(1](2)}(2) -> 4)-alpha-D-Glcp. Acetylation occurs at the O-2 site of mannose. Acetyl substitution degree is 8.21%. The results of structure elucidation showed that LP-1 was a low-molecular weight linear O-acetylated glucomannan. The results of oxidative stress experiments indicate that LP-1 exerts antioxidant effects on HUVEC by activating the Nrf2/HO-1 signaling pathway, thereby improving endothelial cell damage induced by H2O2, and has the potential to be developed as an antioxidant drug.
关键词	Lilium lancifolium Polysaccharide Glucomannan Structural characteristics Oxidative stress
英文关键词	PHYSICOCHEMICAL PROPERTIES; IN-VITRO; IMMUNOMODULATORY ACTIVITY; CHEMICAL-CHARACTERIZATION; SUPEROXIDE-DISMUTASE; POLYSACCHARIDE; DEACETYLATION; EXTRACTION; ACEMANNAN; ANTITUMOR
WOS研究方向	Agriculture, Multidisciplinary
WOS记录号	WOS:001150409400003
来源期刊	CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283253
作者单位	Tibet Agriculture & Animal Husbandry University; Tibet Agriculture & Animal Husbandry University; Tibet Agriculture & Animal Husbandry University; Tibet Agriculture & Animal Husbandry University
推荐引用方式 GB/T 7714	Yuan, Lei,Zhong, ZhengChang,Liu, Yu,et al. Structural characterization of a low-molecular weight linear O-acetyl-glucomannan in Lilium lancifolium from Tibet and its protected H2O2-induced oxidative stress in HUVEC cells[J],2024,11(1).
APA	Yuan, Lei,Zhong, ZhengChang,Liu, Yu,Quan, Hong,&Lan, XiaoZhong.(2024).Structural characterization of a low-molecular weight linear O-acetyl-glucomannan in Lilium lancifolium from Tibet and its protected H2O2-induced oxidative stress in HUVEC cells.CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE,11(1).
MLA	Yuan, Lei,et al."Structural characterization of a low-molecular weight linear O-acetyl-glucomannan in Lilium lancifolium from Tibet and its protected H2O2-induced oxidative stress in HUVEC cells".CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE 11.1(2024).