气候变化领域集成服务门户(CCPortal): Hybridization and divergent climatic preferences drive divergence of two allopatric Gentiana species on the Qinghai-Tibet Plateau

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DOI	10.1093/aob/mcad179
	Hybridization and divergent climatic preferences drive divergence of two allopatric Gentiana species on the Qinghai-Tibet Plateau
	Fu, Peng-Cheng; Twyford, Alex D.; Hao, Yu-Tong; Zhang, Yue; Chen, Shi-Long; Sun, Shan-Shan
发表日期	2023
ISSN	0305-7364
EISSN	1095-8290
英文摘要	Background and Aims Exploring how species diverge is vital for understanding the drivers of speciation. Factors such as geographical separation and ecological selection, hybridization, polyploidization and shifts in mating system are all major mechanisms of plant speciation, but their contributions to divergence are rarely well understood. Here we test these mechanisms in two plant species, Gentiana lhassica and G. hoae, with the goal of understanding recent allopatric species divergence on the Qinghai-Tibet Plateau (QTP).Methods We performed Bayesian clustering, phylogenetic analysis and estimates of hybridization using 561 302 nuclear genomic single nucleotide polymorphisms (SNPs). We performed redundancy analysis, and identified and annotated species-specific SNPs (ssSNPs) to explore the association between climatic preference and genetic divergence. We also estimated genome sizes using flow cytometry to test for overlooked polyploidy.Key Results Genomic evidence confirms that G. lhassica and G. hoae are closely related but distinct species, while genome size estimates show divergence occurred without polyploidy. Gentiana hoae has significantly higher average FIS values than G. lhassica. Population clustering based on genomic SNPs shows no signature of recent hybridization, but each species is characterized by a distinct history of hybridization with congeners that has shaped genome-wide variation. Gentiana lhassica has captured the chloroplast and experienced introgression with a divergent gentian species, while G. hoae has experienced recurrent hybridization with related taxa. Species distribution modelling suggested range overlap in the Last Interglacial Period, while redundancy analysis showed that precipitation and temperature are the major climatic differences explaining the separation of the species. The species differ by 2993 ssSNPs, with genome annotation showing missense variants in genes involved in stress resistance.Conclusions This study suggests that the distinctiveness of these species on the QTP is driven by a combination of hybridization, geographical isolation, mating system differences and evolution of divergent climatic preferences.
关键词	Allopatric hybridization climatic variables Gentiana geographical isolation species pair species-specific SNP
英文关键词	WHOLE-GENOME ASSOCIATION; REPRODUCTIVE ISOLATION; HENGDUAN MOUNTAINS; POPULATION; DIVERSIFICATION; EXPRESSION; INFERENCE; EVOLUTION; ALIGNMENT; SPECIATION
WOS研究方向	Plant Sciences
WOS记录号	WOS:001112257100001
来源期刊	ANNALS OF BOTANY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283218
作者单位	Luoyang Normal University; University of Edinburgh; Chinese Academy of Sciences
推荐引用方式 GB/T 7714	Fu, Peng-Cheng,Twyford, Alex D.,Hao, Yu-Tong,et al. Hybridization and divergent climatic preferences drive divergence of two allopatric Gentiana species on the Qinghai-Tibet Plateau[J],2023.
APA	Fu, Peng-Cheng,Twyford, Alex D.,Hao, Yu-Tong,Zhang, Yue,Chen, Shi-Long,&Sun, Shan-Shan.(2023).Hybridization and divergent climatic preferences drive divergence of two allopatric Gentiana species on the Qinghai-Tibet Plateau.ANNALS OF BOTANY.
MLA	Fu, Peng-Cheng,et al."Hybridization and divergent climatic preferences drive divergence of two allopatric Gentiana species on the Qinghai-Tibet Plateau".ANNALS OF BOTANY (2023).