气候变化领域集成服务门户(CCPortal): Characterization of drought stress-mitigating Rhizobium from faba bean (Vicia faba L.) in the Chinese Qinghai-Tibet Plateau

CCPortal

DOI	10.3389/fmicb.2023.1212996
	Characterization of drought stress-mitigating Rhizobium from faba bean (Vicia faba L.) in the Chinese Qinghai-Tibet Plateau
	Li, Ping; Teng, Changcai; Zhang, Jinfa; Liu, Yujiao; Wu, Xuexia; He, Tao
发表日期	2023
EISSN	1664-302X
卷号	14
英文摘要	Rhizobium-driven symbiotic nitrogen-fixation in legumes not only benefits the growth but also enhances the stress tolerance of plants. Isolating and characterizing efficient, drought-tolerant rhizobia is a central goal for improving crop yields in arid regions. Here, we phylogenetically and biochemically characterized a novel strain of Rhizobium ('QHCD11') sampled from the root nodules of faba beans growing in an arid agricultural area in Qinghai-Tibet. We further tested the drought tolerance of the strain as well as of 'Qingcan 14' faba bean seedlings inoculated with it. Biochemical characterization involved bromothymol blue (BTB) tests, carbon metabolic profiling (Biolog GENIII), DNA-DNA hybridization (dDDH) assays, average nucleotide identity (ANI) analyses, and 16S rRNA sequencing. The result indicated that strain 'QHCD11' likely belongs to the Rhizobium indicum species. Drought stress tolerance was assessed by exposure to polyethylene glycol (PEG-6000) at concentrations of 0, 10, 15, and 20%. Increasing concentrations of PEG-6000 tended to result in decreased growth of 'QHCD11', although the strain performed better at 20% PEG 6000 than at 15%. Inoculation of drought-stressed faba bean seedlings with strain 'QHCD11' improved root vitality, chlorophyll content, antioxidant enzyme activities, and plant height. We suggest that inoculation of faba beans with 'QHCD11' is an environmentally sound strategy for mitigating crop drought stress in arid and semi-arid regions. In addition, the results presents here will benefit future studies into faba bean-rhizobia symbioses under drought stress.
关键词	Rhizobium phylogeny biochemical characterization drought tolerance Vicia faba
英文关键词	PHASEOLUS-VULGARIS; OSMOTIC-STRESS; PLANT-GROWTH; TOLERANCE; DIVERSITY; BACTERIA; STRAINS; SOIL; INOCULATION; ADAPTATION
WOS研究方向	Microbiology
WOS记录号	WOS:001063220100001
来源期刊	FRONTIERS IN MICROBIOLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283212
作者单位	Qinghai University; Qinghai University; Qinghai University
推荐引用方式 GB/T 7714	Li, Ping,Teng, Changcai,Zhang, Jinfa,et al. Characterization of drought stress-mitigating Rhizobium from faba bean (Vicia faba L.) in the Chinese Qinghai-Tibet Plateau[J],2023,14.
APA	Li, Ping,Teng, Changcai,Zhang, Jinfa,Liu, Yujiao,Wu, Xuexia,&He, Tao.(2023).Characterization of drought stress-mitigating Rhizobium from faba bean (Vicia faba L.) in the Chinese Qinghai-Tibet Plateau.FRONTIERS IN MICROBIOLOGY,14.
MLA	Li, Ping,et al."Characterization of drought stress-mitigating Rhizobium from faba bean (Vicia faba L.) in the Chinese Qinghai-Tibet Plateau".FRONTIERS IN MICROBIOLOGY 14(2023).