气候变化领域集成服务门户(CCPortal): Metagenomic surveillance of antibiotic resistome in influent and effluent of wastewater treatment plants located on the Qinghai-Tibetan Plateau

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DOI	10.1016/j.scitotenv.2023.162031
	Metagenomic surveillance of antibiotic resistome in influent and effluent of wastewater treatment plants located on the Qinghai-Tibetan Plateau
	Shi, Bin; Zhao, Renxin; Su, Guijin; Liu, Bingyue; Liu, Wenxiu; Xu, Jian; Li, Qianqian; Meng, Jing
发表日期	2023
ISSN	0048-9697
EISSN	1879-1026
卷号	870
英文摘要	As hotspots for the dissemination of antibiotic resistance genes (ARGs), wastewater treatment plants (WWTPs) have attracted global attention. However, there lacks a sufflcient metagenomic surveillance of antibiotic resistome in the WWTPs located on the Qinghai-Tibet Plateau. Here, metagenomic approaches were used to comprehensively investi-gate the occurrence, mobility potential, and bacterial hosts of ARGs in influent and effluent of 18 WWTPs located on the Qinghai-Tibet Plateau. The total ARG relative abundances and diversity were signiflcantly decreased from influent to effluent across the WWTPs. Multidrug, bacitracin, sulfonamide, aminoglycoside, and beta-lactam ARGs generally consisted of the main ARG types in effluent samples, which were distinct from influent samples. A group of 72 core ARGs accounting for 61.8-95.8 % of the total ARG abundances were shared by all samples. Clinically relevant ARGs mainly conferring resistance to beta-lactams were detected in influent (277 ARGs) and effluent (178 ARGs). Metagenomic assembly revealed that the genetic location of an ARG on a plasmid or a chromosome was related to its corresponding ARG type, demonstrating the distinction in the mobility potential of different ARG types. The abun-dance of plasmid-mediated ARGs accounted for a much higher proportion than that of chromosome-mediated ARGs in both influent and effluent. Moreover, the ARGs co-occurring with diverse mobile genetic elements in the effluent ex-hibited a comparable mobility potential with the influent. Furthermore, 137 metagenome-assembled genomes (MAGs) assigned to 13 bacterial phyla were identifled as the ARG hosts, which could be effectively treated in most WWTPs. Notably, 46 MAGs were found to carry multiple ARG types and the potential pathogens frequently exhibited multi-antibiotic resistance. Some ARG types tended to be carried by certain bacteria, showing a speciflc host-resistance association pattern. This study highlights the necessity for metagenomic surveillance and will facilitate risk assessment and control of antibiotic resistome in WWTPs located on the vulnerable area.
关键词	Antibiotic resistance genes Wastewater Mobility Bacterial hosts Metagenomics Qinghai-Tibet Plateau
英文关键词	RESISTANCE GENES; ANTIMICROBIAL RESISTANCE; SEWAGE; TRANSMISSION; ENVIRONMENT; BACTERIA; HOTSPOTS; HOSTS
WOS研究方向	Environmental Sciences
WOS记录号	WOS:000935413700001
来源期刊	SCIENCE OF THE TOTAL ENVIRONMENT
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283127
作者单位	Chinese Academy of Sciences; Central China Normal University; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Chinese Research Academy of Environmental Sciences; Chinese Research Academy of Environmental Sciences; Chinese Research Academy of Environmental Sciences; Central China Normal University
推荐引用方式 GB/T 7714	Shi, Bin,Zhao, Renxin,Su, Guijin,et al. Metagenomic surveillance of antibiotic resistome in influent and effluent of wastewater treatment plants located on the Qinghai-Tibetan Plateau[J],2023,870.
APA	Shi, Bin.,Zhao, Renxin.,Su, Guijin.,Liu, Bingyue.,Liu, Wenxiu.,...&Meng, Jing.(2023).Metagenomic surveillance of antibiotic resistome in influent and effluent of wastewater treatment plants located on the Qinghai-Tibetan Plateau.SCIENCE OF THE TOTAL ENVIRONMENT,870.
MLA	Shi, Bin,et al."Metagenomic surveillance of antibiotic resistome in influent and effluent of wastewater treatment plants located on the Qinghai-Tibetan Plateau".SCIENCE OF THE TOTAL ENVIRONMENT 870(2023).