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DOI	10.1111/gfs.12646
	Evaluation of chromosome diversity of Elymus nutans on the Qinghai-Tibet plateau by FISH
	Liu, Ruijuan; Jiang, Liling; Liu, Demei; Shen, Jicheng; Chen, Wenjie
发表日期	2023
ISSN	0142-5242
EISSN	1365-2494
英文摘要	Elymus nutans Griseb. is an important herbage for forage and ecological restoration in the Qinghai-Tibet Plateau (QTP). It is widely distributed in different habitats of the QTP and has been reported to have high genetic diversity in protein, morphology and DNA sequence. The main objective of our investigation has been to obtain some knowledge of E. nutans chromosome genetic diversity. In this study, the TE probe S5 and tandem repeat probe AAG combined with sequential GISH were able to successfully distinguish different subgenomes and chromosomes in 7 E. nutans accessions from major global distribution sites. On this basis, a total of 123 individuals from four representative populations of E. nutans in the QTP were hybridised. The results of the experiment showed that four types of reciprocal translocations between subgenomes were observed in the four wild populations, and the translocation chromosomes, frequency, and heterozygosis were different in each population. The distribution and quantity of S5 and AAG signals also varied within and among the four populations. The results indicated that E. nutans had high genetic diversity at the chromosome level both within and among populations, and it was speculated that non-homologous recombination was one of the reasons for the high genetic diversity. These results provide important theoretical guidance for the collection, protection, and evaluation of E. nutans.
关键词	Elymus nutans FISH genetic diversity non-homologous recombination translocation
英文关键词	GENETIC DIVERSITY; POACEAE TRITICEAE; GENERA
WOS研究方向	Agronomy
WOS记录号	WOS:001128826400001
来源期刊	GRASS AND FORAGE SCIENCE
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283117
作者单位	Chinese Academy of Sciences; Chinese Academy of Sciences; Qinghai University
推荐引用方式 GB/T 7714	Liu, Ruijuan,Jiang, Liling,Liu, Demei,et al. Evaluation of chromosome diversity of Elymus nutans on the Qinghai-Tibet plateau by FISH[J],2023.
APA	Liu, Ruijuan,Jiang, Liling,Liu, Demei,Shen, Jicheng,&Chen, Wenjie.(2023).Evaluation of chromosome diversity of Elymus nutans on the Qinghai-Tibet plateau by FISH.GRASS AND FORAGE SCIENCE.
MLA	Liu, Ruijuan,et al."Evaluation of chromosome diversity of Elymus nutans on the Qinghai-Tibet plateau by FISH".GRASS AND FORAGE SCIENCE (2023).