气候变化领域集成服务门户(CCPortal): Antigenic variation in hemagglutinin-neuraminidase of Newcastle disease virus isolated from Tibet, China

CCPortal

DOI	10.1016/j.vetmic.2023.109872
	Antigenic variation in hemagglutinin-neuraminidase of Newcastle disease virus isolated from Tibet, China
	Chang, Zhengwu; Dong, Xiaoyu; Guan, Zhao; Lu, Kejia; Chen, Xi; Wei, Xi; Guo, Hanwei; Dang, Ruyi; Wang, Juan; Wang, Xinglong; Xiao, Sa; Yang, Zengqi; Liu, Haijin
发表日期	2023
ISSN	0378-1135
EISSN	1873-2542
卷号	285
英文摘要	Vaccines are widely used to prevent Newcastle disease virus (NDV). Under the pressure of immunization, NDVs with mutations among epitopes of F and HN protein were isolated, which indicates that the efficiency of vaccine may decrease in terms of preventing emerged NDV. However, the lack of evidences to support whether these mutations contribute to antigenic mutation and immune escape in NDV leading to the controversy that the matched vaccine is more effective than the mismatched vaccine. In this study, a genotype VII velogenic NDV strain (C22) was isolated from a vaccinated farm in Tibet, China. We found that this strain was close to NDV from east China, but it had a specific mutation (K138R) in one epitope (131DYIGGIGKE139) of HN protein. This mutation might change the interaction between amino acids in stalk-head link region of HN protein and then induce the specific antibody to worse recognize the C22 strain, but it did not alter viral virulence and growth ability. Then, the C22 strain was attenuated via modification of the F protein cleavage site to generate a matched vaccine. Comparing to a mismatched vaccine (LaSota), this matched vaccine showed advantages in inhibiting viral shedding and tissue damage. However, both vaccines induced chicken to generate similar level of neutralizing antibodies against C22, C22mut (R138K) and LaSota. These results suggest that the epitope mutation is insufficient to help NDV escaping neutralizing antibodies of vaccinated chicken, supporting that the merits of NDV matched vaccine are not totally related to humoral immunity.
关键词	Newcastle Disease Virus HN protein Epitope Vaccine Neutralizing antibody
英文关键词	EPITOPE; GENE; GLYCOPROTEIN; EFFICACY
WOS研究方向	Microbiology ; Veterinary Sciences
WOS记录号	WOS:001086493700001
来源期刊	VETERINARY MICROBIOLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283105
作者单位	Northwest A&F University - China; Southwest Minzu University
推荐引用方式 GB/T 7714	Chang, Zhengwu,Dong, Xiaoyu,Guan, Zhao,et al. Antigenic variation in hemagglutinin-neuraminidase of Newcastle disease virus isolated from Tibet, China[J],2023,285.
APA	Chang, Zhengwu.,Dong, Xiaoyu.,Guan, Zhao.,Lu, Kejia.,Chen, Xi.,...&Liu, Haijin.(2023).Antigenic variation in hemagglutinin-neuraminidase of Newcastle disease virus isolated from Tibet, China.VETERINARY MICROBIOLOGY,285.
MLA	Chang, Zhengwu,et al."Antigenic variation in hemagglutinin-neuraminidase of Newcastle disease virus isolated from Tibet, China".VETERINARY MICROBIOLOGY 285(2023).