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DOI	10.1186/s12934-023-02018-0
	Nonribosomal antibacterial peptides isolated from Streptomyces agglomeratus 5-1-3 in the Qinghai-Tibet Plateau
	Jiang, Kan; Chen, Ximing; Zhang, Wei; Guo, Yehong; Liu, Guangxiu
发表日期	2023
EISSN	1475-2859
卷号	22 期号:1
英文摘要	Background New antibiotics are urgently needed in clinical treatment of superdrug-resistant bacteria. Nonribosomal peptides (NRPs) are a major source of antibiotics because they exhibit structural diversity, and unique antibacterial mechanisms and resistance. Analysis of gene clusters of S. agglomeratus 5-1-3 showed that Clusters 3, 6, 12, 21, and 28 were used to synthesize NRPs. Here, we examined secondary metabolites of S. agglomeratus 5-1-3 isolated from soils in the Qinghai-Tibet Plateau, China, for NRPs with antibacterial activity. Results We isolated a total of 36 Streptomyces strains with distinct colony morphological characteristics from 7 soil samples. We screened 8 Streptomyces strains resistant to methicillin-resistant Staphylococcus aureus (MRSA). We then selected S. agglomeratus 5-1-3 for further study based on results of an antibacterial activity test. Here, we isolated three compounds from S. agglomeratus 5-1-3 and characterized their properties. The crude extract was extracted with ethyl acetate and purified with column chromatography and semipreparative high-performance liquid chromatogra-phy (HPLC). We characterized the three compounds using NMR analyses as echinomycin (1), 5,7,4'-trihydroxy-3.3 ',5 '- trimethoxy flavone (2), and 2,6,2 ', 6 '-tetramethoxy-4,4-bis(2,3-epoxy-1-hydroxypropyl)-biphenyl (3). We tested the antibacterial activity of pure compounds from strain 5-1-3 with the Oxford cup method. NRP echinomycin (1) showed excellent anti-MRSA activity with a minimum inhibitory concentration (MIC) of 2.0 mu g/mL. Meanwhile, MIC of com-pound 2 and 3 was 128.0 mu g/mL for both. In addition, 203 mg of echinomycin was isolated from 10 L of the crude extract broth of strain 5-1-3. Conclusion In this study, S. agglomeratus 5-1-3 with strong resistance to MRSA was isolated from the soils in the Qinghai-Tibet Plateau. Strain 5-1-3 had a high yield of echinomycin (1) an NRP with a MIC of 2 mu g/mL against MRSA. We propose that echinomycin derived from S. agglomeratus 5-1-3 may be a potent antibacterial agent for pharma-ceutical use.
关键词	S. agglomeratus 5-1-3 Antibacterial Echinomycin Nonribosomal peptides MRSA
英文关键词	SP NOV.; STREPTOMYCES; IDENTIFICATION; ANTIBIOTICS; RESISTANCE; EXPRESSION; STRAIN
WOS研究方向	Biotechnology & Applied Microbiology
WOS记录号	WOS:000909950400001
来源期刊	MICROBIAL CELL FACTORIES
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283086
作者单位	Gansu Agricultural University; Chinese Academy of Sciences
推荐引用方式 GB/T 7714	Jiang, Kan,Chen, Ximing,Zhang, Wei,et al. Nonribosomal antibacterial peptides isolated from Streptomyces agglomeratus 5-1-3 in the Qinghai-Tibet Plateau[J],2023,22(1).
APA	Jiang, Kan,Chen, Ximing,Zhang, Wei,Guo, Yehong,&Liu, Guangxiu.(2023).Nonribosomal antibacterial peptides isolated from Streptomyces agglomeratus 5-1-3 in the Qinghai-Tibet Plateau.MICROBIAL CELL FACTORIES,22(1).
MLA	Jiang, Kan,et al."Nonribosomal antibacterial peptides isolated from Streptomyces agglomeratus 5-1-3 in the Qinghai-Tibet Plateau".MICROBIAL CELL FACTORIES 22.1(2023).