气候变化领域集成服务门户(CCPortal): Molecular characteristics and pathogenicity of a Tibet-origin mutant avian leukosis virus subgroup J isolated from Tibetan chickens in China

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DOI	10.1016/j.meegid.2023.105415
	Molecular characteristics and pathogenicity of a Tibet-origin mutant avian leukosis virus subgroup J isolated from Tibetan chickens in China
	Cheng, Xiangyu; Yang, Jianhao; Bi, Xiaoqing; Yang, Qi; Zhou, Defang; Zhang, Shicheng; Ding, Longying; Wang, Kang; Hua, Shuhan; Cheng, Ziqiang
发表日期	2023
ISSN	1567-1348
EISSN	1567-7257
卷号	109
英文摘要	Tibetan chicken is found in China Tibet (average altitude; >4500 m). However, little is known about avian leukosis virus subgroup J (ALV-J) found in Tibetan chickens. ALV-J is a typical alpharetrovirus that causes immunosuppression and myelocytomatosis and thus seriously affects the development of the poultry industry. In this study, Tibet-origin mutant ALV-J was isolated from Tibetan chickens and named RKZ-1-RKZ-5. A Myelo-cytomatosis outbreak occurred in a commercial Tibetan chicken farm in Shigatse of Rikaze, Tibet, China, in March 2022. About 20% of Tibetan chickens in the farm showed severe immunosuppression, and mortality increased to 5.6%. Histopathological examination showed typical myelocytomas in various tissues. Virus isolation and phylogenetic analysis demonstrated that ALV-J caused the disease. Gene-wide phylogenetic anal-ysis showed the RKZ isolates were the original strains of the previously reported Tibetan isolates (TBC-J4 and TBC-J6) (identity; 94.5% to 94.9%). Furthermore, significant nucleotide mutations and deletions occurred in the hr1 and hr2 hypervariable regions of gp85 gene, 3'UTR, Y Box, and TATA Box of 3'LTR. Pathogenicity exper-iments demonstrated that the viral load, viremia, and viral shedding level were significantly higher in RKZ-1-infected chickens than in NX0101-infected chickens. Notably, RKZ-1 caused more severe cardiopulmonary damage in SPF chickens. These findings prove the origin of Tibet ALV-J and provide insights into the molecular characteristics and pathogenic ability of ALV-J in the plateau area. Therefore, this study may provide a basis for ALV-J prevention and eradication in Tibet.
关键词	Tibetan chicken Avian leukosis virus subgroup J Sequence analysis Pathogenic analysis
英文关键词	HIGH-ALTITUDE ADAPTATION; ALV-J; PHYLOGENETIC ANALYSIS; DISEASE VIRUS; GENOME; IDENTIFICATION; SEQUENCES; STRAINS; CONTRIBUTES; INFECTION
WOS研究方向	Infectious Diseases
WOS记录号	WOS:001055896700001
来源期刊	INFECTION GENETICS AND EVOLUTION
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283011
作者单位	Shandong Agricultural University; Inner Mongolia Agricultural University
推荐引用方式 GB/T 7714	Cheng, Xiangyu,Yang, Jianhao,Bi, Xiaoqing,et al. Molecular characteristics and pathogenicity of a Tibet-origin mutant avian leukosis virus subgroup J isolated from Tibetan chickens in China[J],2023,109.
APA	Cheng, Xiangyu.,Yang, Jianhao.,Bi, Xiaoqing.,Yang, Qi.,Zhou, Defang.,...&Cheng, Ziqiang.(2023).Molecular characteristics and pathogenicity of a Tibet-origin mutant avian leukosis virus subgroup J isolated from Tibetan chickens in China.INFECTION GENETICS AND EVOLUTION,109.
MLA	Cheng, Xiangyu,et al."Molecular characteristics and pathogenicity of a Tibet-origin mutant avian leukosis virus subgroup J isolated from Tibetan chickens in China".INFECTION GENETICS AND EVOLUTION 109(2023).