气候变化领域集成服务门户(CCPortal): Arthrobacter caoxuetaonis sp. nov., Arthrobacter zhangbolii sp. nov. and Arthrobacter gengyunqii sp. nov., isolated from Marmota himalayana faeces from Qinghai- Tibet Plateau

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DOI	10.1099/ijsem.0.005742
	Arthrobacter caoxuetaonis sp. nov., Arthrobacter zhangbolii sp. nov. and Arthrobacter gengyunqii sp. nov., isolated from Marmota himalayana faeces from Qinghai- Tibet Plateau
	Liu, Yue; Zhang, Gui; Yang, Jing; Cheng, Yanpeng; Ye, Lin; Lai, Xin-He; Yang, Caixin; Ma, Caiyun; Tao, Yuanmeihui; Jin, Dong; Lu, Shan; Liu, Liyun; Xu, Jianguo
发表日期	2023
ISSN	1466-5026
EISSN	1466-5034
卷号	73 期号:4
英文摘要	Six aerobic or facultative anaerobic, motile, Gram- stain- positive, catalase- positive and oxidase- negative strains (zg- Y453T, zgY324, zg-Y462T, zg-Y411, zg-Y809T and zg- Y786) were isolated from different faecal samples of Marmota himalayana from the Qinghai-Tibet Plateau. Pale yellow, round, raised and moist colonies appeared 48 h after incubation at 28 degrees C on brain-heart infusion plates supplemented with 5 % defibrinated sheep blood. According to the 16S rRNA gene sequence alignment, two strain pairs (zg- Y453T/zg-Y324 and zg-Y462T/zg- Y411) shared the highest similarities to Arthrobacter luteolus (99.5 and 99.2 %), and the other one (zg- Y809T/zg-Y786) to Arthrobacter citreus (99.5 %). Results of phylogenetic analysis based on the 16S rRNA gene and genome sequences showed that these six strains represented three separate species within the genus Arthrobacter. The average nucleotide identity and digital DNA-DNA hybridization values between the three novel type strains (zg- Y453T/ zg-Y462T/zg-Y809T) and other known species in this genus were all below respective thresholds (70.2-81.5/19.6-24.2 %, 70.6- 81.8/19.8-25.0 %, and 70.4-88.2/19.9-35.3 %). Although phylogenetically related, there were obvious chemotaxonomic and phenotypic differences: strain pair zg- Y462T/zg-Y411 had anteiso- C15: 0 as the only major fatty acid; the three novel species had different dominant quinones, MK- 8(H2) in strains zg- Y462T/zg-Y809T (74.8/81.1 %) and MK- 8(H2)/MK-9(H2) (43.1/53.0 %) in zgY453T; similarly, the ability to reduce nitrate in strains zg- Y453T and zg- Y462T could differentiate them from zg- Y809T. All strains had diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol, but differed slightly in the types of unidentified glycolipids, phospholipids and lipids. Based on the results of these polyphasic taxonomic analyses, three novel species within the genus Arthrobacter are proposed, namely Arthrobacter caoxuetaonis sp. nov. (type strain, zg- Y453T=GDMCC 1.2809T=JCM 35173T), Arthrobacter zhangbolii sp. nov. (type strain, zg- Y462T=GDMCC 1.2880T=JCM 35170T) and Arthrobacter gengyunqii sp. nov. (type strain, zg- Y809T=GDMCC 1.2808T=JCM 35168T).
关键词	Arthrobacter Qinghai-Tibet Plateau novel species polyphasic taxonomic method
英文关键词	MULTIPLE SEQUENCE ALIGNMENT; EMENDED DESCRIPTION; MAXIMUM-LIKELIHOOD; GENUS ARTHROBACTER; TREES; MENAQUINONES; BACTERIUM; DISTANCE; STRAINS
WOS研究方向	Microbiology
WOS记录号	WOS:000976043900019
来源期刊	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/282912
作者单位	Shanxi Medical University; Chinese Center for Disease Control & Prevention; National Institute for Communicable Disease Control & Prevention, Chinese Center for Disease Control & Prevention; Shandong First Medical University & Shandong Academy of Medical Sciences; Chinese Academy of Medical Sciences - Peking Union Medical College; Shenzhen Center for Disease Control & Prevention (SZCDC); Shangqiu Normal University; Nankai University
推荐引用方式 GB/T 7714	Liu, Yue,Zhang, Gui,Yang, Jing,et al. Arthrobacter caoxuetaonis sp. nov., Arthrobacter zhangbolii sp. nov. and Arthrobacter gengyunqii sp. nov., isolated from Marmota himalayana faeces from Qinghai- Tibet Plateau[J],2023,73(4).
APA	Liu, Yue.,Zhang, Gui.,Yang, Jing.,Cheng, Yanpeng.,Ye, Lin.,...&Xu, Jianguo.(2023).Arthrobacter caoxuetaonis sp. nov., Arthrobacter zhangbolii sp. nov. and Arthrobacter gengyunqii sp. nov., isolated from Marmota himalayana faeces from Qinghai- Tibet Plateau.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,73(4).
MLA	Liu, Yue,et al."Arthrobacter caoxuetaonis sp. nov., Arthrobacter zhangbolii sp. nov. and Arthrobacter gengyunqii sp. nov., isolated from Marmota himalayana faeces from Qinghai- Tibet Plateau".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 73.4(2023).