气候变化领域集成服务门户(CCPortal): Inflorescence Trait Diversity and Genotypic Differentiation as Influenced by the Environment in Elymus nutans Griseb. from Qinghai-Tibet Plateau

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DOI	10.3390/agronomy13041004
	Inflorescence Trait Diversity and Genotypic Differentiation as Influenced by the Environment in Elymus nutans Griseb. from Qinghai-Tibet Plateau
	Li, Jin; Tian, Haoqi; Ji, Wenqin; Zhang, Changbing; Chen, Shiyong
发表日期	2023
EISSN	2073-4395
卷号	13 期号:4
英文摘要	The alpine forage grass species Elymus nutans Griseb. is widely distributed in the Qinghai-Tibet Plateau and the Himalayas due to its high adaptability. However, it has become threatened by climate warming and excessive grazing. Thus, understanding its genetic and phenotypic information is crucial to aid resource management and conservation efforts. In this study, microsatellite markers were developed based on RNA-seq transcriptome data from E. nutans Griseb. varieties 'Aba' and 'Kangbei', resulting in the identification of 48,457 SSRs from 304,554 de novo assembled unigenes. Seventeen polymorphic markers, 13 inflorescence phenotypic traits, and seed shattering values were determined for 31 E. nutans accessions collected from eastern Tibet. The molecular markers generated 134 well-amplified bands with a mean Nei's genetic diversity of 0.31 and a Shannon information index of 0.46. Pairwise genetic similarity ranged from 0.554 to 0.895, with an average of 0.729. Based on the molecular marker data, all accessions were divided into two classes via the unweighted pair-group method with arithmetic mean (UPGMA), the Markov chain Monte Carlo method, and the principal coordinate analysis (PCA) method. We used Tassel analysis to determine 11 loci with a significant relationship to phenotypic traits, and Pearson's correlation analysis showed that some inflorescence traits were significantly influenced by the environment. Furthermore, we detected strong patterns of isolation by both environment (IBE) and distance (IBD) via Mantel analysis. This study provides valuable insights into the genetic and phenotypic differentiation of E. nutans, informing germplasm resource evaluation and future breeding.
关键词	Elymus nutans Qinghai-Tibet plateau transcriptome genetic diversity phenotypic differentiation isolation patterns
英文关键词	SSR-MARKERS; ASSOCIATION ANALYSIS; GENETIC DIVERSITY; POACEAE TRITICEAE; DIVERGENCE; SOFTWARE; QTL
WOS研究方向	Agronomy ; Plant Sciences
WOS记录号	WOS:000978342700001
来源期刊	AGRONOMY-BASEL
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/282767
作者单位	Southwest Minzu University; Southwest Minzu University; Qinghai University
推荐引用方式 GB/T 7714	Li, Jin,Tian, Haoqi,Ji, Wenqin,et al. Inflorescence Trait Diversity and Genotypic Differentiation as Influenced by the Environment in Elymus nutans Griseb. from Qinghai-Tibet Plateau[J],2023,13(4).
APA	Li, Jin,Tian, Haoqi,Ji, Wenqin,Zhang, Changbing,&Chen, Shiyong.(2023).Inflorescence Trait Diversity and Genotypic Differentiation as Influenced by the Environment in Elymus nutans Griseb. from Qinghai-Tibet Plateau.AGRONOMY-BASEL,13(4).
MLA	Li, Jin,et al."Inflorescence Trait Diversity and Genotypic Differentiation as Influenced by the Environment in Elymus nutans Griseb. from Qinghai-Tibet Plateau".AGRONOMY-BASEL 13.4(2023).