气候变化领域集成服务门户(CCPortal): Flavonoid synthesis in Lamiophlomis rotata from Qinghai-Tibet Plateau is influenced by soil properties, microbial community, and gene expression

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DOI	10.1016/j.jplph.2023.154043
	Flavonoid synthesis in Lamiophlomis rotata from Qinghai-Tibet Plateau is influenced by soil properties, microbial community, and gene expression
	Qiao, Feng; Lu, Yueheng; Geng, Guigong; Zhou, Lianyu; Chen, Zhenning; Wang, Luhao; Xie, Huichun; Qiu, Quan-Sheng
发表日期	2023
ISSN	0176-1617
EISSN	1618-1328
卷号	287
英文摘要	Lamiophlomis rotata is a medicinal plant in Qinghai-Tibet Plateau, in which flavonoid compounds are the major medicinal components. However, it remains unclear how flavonoid metabolism of L. rotata is influenced by soil properties and microbial community. In this study, we collected L. rotata seedlings and rhizosphere soils from five habitats ranging from 3750 to 4270 m of altitude and analyzed the effects of habitat conditions on flavonoid metabolism. The activities of peroxidase, cellulase, and urease were increased with altitude, while those of alkaline phosphatase, alkaline protease, and sucrase were decreased with altitude. Analysis of OTUs showed that the total number of bacterial genera was higher than that of fungal genera. The highest number of fungal genera was 132, and that of bacterial genera was 33 in Batang (BT) town in Yushu County at an altitude of 3880 m, suggesting that the fungal communities may play a critical role in L. rotata rhizosphere soils. Flavonoids in leaves and roots of L. rotata shared a similar pattern, with a trend of increasing levels with altitude. The highest flavonoid content measured, 12.94 mg/g in leaves and 11.43 mg/g in roots, was from Zaduo (ZD) County at an altitude of 4208 m. Soil peroxidases affected quercetin content in leaves of L. rotata, while the fungus Sebacina affected flavonoid content in leaves and roots of L. rotata. The expression of PAL, F3 & PRIME;H, FLS, and FNS genes showed a declining trend in leaves with altitude, while F3H showed an increasing trend in both leaves and roots. Overall, soil physicochemical properties and microbial community affect flavonoid metabolism in L. rotata in Qinghai-Tibet Plateau. The variations in flavonoid content and gene expression as well as their associations with soil factors revealed the complexity of the growth conditions and genetic makeup in L. rotata habitats of QinghaiTibet Plateau.
关键词	Lamiophlomis rotata Flavonoids Soil physicochemical properties Soil microbial community Gene expression Qinghai -Tibet Plateau
英文关键词	IDENTIFICATION; ACCUMULATION; QUALITY
WOS研究方向	Plant Sciences
WOS记录号	WOS:001034241900001
来源期刊	JOURNAL OF PLANT PHYSIOLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/282700
作者单位	Qinghai Normal University; Qinghai Normal University; Qinghai Normal University; Qinghai University; Lanzhou University; Guangdong Ocean University; Lanzhou University; Qinghai Normal University; Lanzhou University
推荐引用方式 GB/T 7714	Qiao, Feng,Lu, Yueheng,Geng, Guigong,et al. Flavonoid synthesis in Lamiophlomis rotata from Qinghai-Tibet Plateau is influenced by soil properties, microbial community, and gene expression[J],2023,287.
APA	Qiao, Feng.,Lu, Yueheng.,Geng, Guigong.,Zhou, Lianyu.,Chen, Zhenning.,...&Qiu, Quan-Sheng.(2023).Flavonoid synthesis in Lamiophlomis rotata from Qinghai-Tibet Plateau is influenced by soil properties, microbial community, and gene expression.JOURNAL OF PLANT PHYSIOLOGY,287.
MLA	Qiao, Feng,et al."Flavonoid synthesis in Lamiophlomis rotata from Qinghai-Tibet Plateau is influenced by soil properties, microbial community, and gene expression".JOURNAL OF PLANT PHYSIOLOGY 287(2023).