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DOI	10.1016/j.envres.2023.116233
	Regional distribution and migration potential of antibiotic resistance genes in croplands of Qinghai Tibet Plateau
	Song, Dagang; Tang, Xue; Tariq, Akash; Pan, Kaiwen; Li, Dong
发表日期	2023
ISSN	0013-9351
EISSN	1096-0953
卷号	231
英文摘要	Agricultural activities have recently disturbed the ecosystem of the Qinghai-Tibet Plateau and the shift of antibiotic resistance genes (ARGs) in the different types of farmlands is not well understood, so more compre-hensive ecological barrier management measures cannot be provided for the region. This research was performed to exploring ARG pollution in cropland soil on the Qinghai-Tibet Plateau to obtain information on the geographical and climatic factors shaping the ARG distribution. Based on high-throughput quantitative PCR (HT-qPCR) analysis, the ARG abundance in farmland ranged from 5.66 x 105 to 6.22 x 107 copies per gram of soil higher than previous research at soil and wetland in Qinghai-Tibet plateau, and it was higher in wheat and barley soils than in corn soil. The distribution of ARGs exhibited regional features as ARG abundance was adversely affected by mean annual precipitation and temperature with lower temperature and less rainfall at high altitude. According to network analysis and structural equation modeling (SEM), mobile genetic elements (MGEs) and heavy metals are the key drivers of ARG dissemination on the Qinghai-Tibet Plateau as they show negative relationship with ARGs, and selection copressure from heavy metals in cropland soil increases the horizontal gene transfer (HGT) potential of ARGs through synergistic selection effects, each contribution to the ARGs was 19% and 29% respectively. This research suggests the need to focus on controlling heavy metals and MGEs to constrain the dissemination of ARGs, as arable soil is already slightly contaminated by heavy metals.
关键词	Antibiotic resistance genes Qinghai-Tibet Plateau Cropland soil Mobile genetic element
英文关键词	BACTERIAL COMMUNITIES; AGRICULTURAL SOILS; TETRACYCLINE; DIVERSITY; MANURE; INCREASES; RESISTOME; INTEGRONS; POLLUTION; METALS
WOS研究方向	Environmental Sciences ; Public, Environmental & Occupational Health
WOS记录号	WOS:001012525100001
来源期刊	ENVIRONMENTAL RESEARCH
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/282617
作者单位	Ministry of Agriculture & Rural Affairs; Chinese Academy of Agricultural Sciences; Biogas Institute of Ministry of Agriculture, CAAS; Chinese Academy of Sciences; Chinese Academy of Sciences; Chengdu Institute of Biology, CAS; Chinese Academy of Sciences; Xinjiang Institute of Ecology & Geography, CAS; Chinese Academy of Sciences; Chengdu Institute of Biology, CAS
推荐引用方式 GB/T 7714	Song, Dagang,Tang, Xue,Tariq, Akash,et al. Regional distribution and migration potential of antibiotic resistance genes in croplands of Qinghai Tibet Plateau[J],2023,231.
APA	Song, Dagang,Tang, Xue,Tariq, Akash,Pan, Kaiwen,&Li, Dong.(2023).Regional distribution and migration potential of antibiotic resistance genes in croplands of Qinghai Tibet Plateau.ENVIRONMENTAL RESEARCH,231.
MLA	Song, Dagang,et al."Regional distribution and migration potential of antibiotic resistance genes in croplands of Qinghai Tibet Plateau".ENVIRONMENTAL RESEARCH 231(2023).