气候变化领域集成服务门户(CCPortal): The Enhanced Affinity of WRKY Reinforces Drought Tolerance in Solanum lycopersicum L.: An Innovative Bioinformatics Study

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DOI	10.3390/plants12040762
	The Enhanced Affinity of WRKY Reinforces Drought Tolerance in Solanum lycopersicum L.: An Innovative Bioinformatics Study
	Debnath, Sandip; Kant, Achal; Bhowmick, Pradipta; Malakar, Ayushman; Purkaystha, Shampa; Jena, Binod Kumar; Mudgal, Gaurav; Rahimi, Mehdi; Helal, Md Mostofa Uddin; Hasan, Rakibul; Chen, Jen-Tsung; Azam, Faizul
发表日期	2023
EISSN	2223-7747
卷号	12 期号:4
英文摘要	In the scenario of global climate change, understanding how plants respond to drought is critical for developing future crops that face restricted water resources. This present study focuses on the role of WRKY transcription factors on drought tolerance in tomato, Solanum lycopersicum L., which is a significant vegetable crop. WRKY transcription factors are a group of proteins that regulate a wild range of growth and developmental processes in plants such as seed germination and dormancy and the stress response. These transcription factors are defined by the presence of a DNA-binding domain, namely, the WRKY domain. It is well-known that WRKY transcription factors can interact with a variety of proteins and therefore control downstream activities. It aims to simulate the effect of curcumin, a bioactive compound with regulatory capacity, on the protein-protein interaction events by WRKY transcription factors with an emphasis on drought stress. It was found that curcumin binds to WRKY with an energy of -11.43 kcal/mol with inhibitory concentration (K-i) 0.12 mM and has the potential to improve fruit quality and reinforce drought tolerance of S. lycopersicum, according to the results based on bioinformatics tools. The root means square deviation (RMSD) of the C-alpha, the backbone of 2AYD with ligand coupled complex, displayed a very stable structure with just a little variation of 1.89 angstrom. MD simulation trajectory of C alpha atoms of 2AYD bound to Curcumin revealed more un-ordered orientation in PC1 and PC10 modes and more toward negative correlation from the initial 400 frames during PCA. Establishing the binding energies of the ligand-target interaction is essential in order to characterize the compound's binding affinity to the drought transcription factor. We think we have identified a phyto-agent called curcumin that has the potential to enhance the drought tolerance. Compared to the part of the mismatch repair-base technique that can be used to fix drought related genes, curcumin performed better in a drop-in crop yield over time, and it was suggested that curcumin is a potential candidate factor for improving drought tolerance in tomatoes, and it needs future validation by experiments in laboratory and field.
英文关键词	phytochemicals; drought stress; bioinformatics; molecular docking; tomato; curcumin
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000940905700001
来源期刊	PLANTS-BASEL
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/281598
作者单位	Visva Bharati University; Centurion University of Technology & Management; Chandigarh University; Graduate University of Advanced Technology; Shanxi Agricultural University; Sylhet Agricultural University; National University Kaohsiung; Qassim University
推荐引用方式 GB/T 7714	Debnath, Sandip,Kant, Achal,Bhowmick, Pradipta,et al. The Enhanced Affinity of WRKY Reinforces Drought Tolerance in Solanum lycopersicum L.: An Innovative Bioinformatics Study[J],2023,12(4).
APA	Debnath, Sandip.,Kant, Achal.,Bhowmick, Pradipta.,Malakar, Ayushman.,Purkaystha, Shampa.,...&Azam, Faizul.(2023).The Enhanced Affinity of WRKY Reinforces Drought Tolerance in Solanum lycopersicum L.: An Innovative Bioinformatics Study.PLANTS-BASEL,12(4).
MLA	Debnath, Sandip,et al."The Enhanced Affinity of WRKY Reinforces Drought Tolerance in Solanum lycopersicum L.: An Innovative Bioinformatics Study".PLANTS-BASEL 12.4(2023).