气候变化领域集成服务门户(CCPortal): Gene Editing for Plant Resistance to Abiotic Factors: A Systematic Review

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DOI	10.3390/plants12020305
	Gene Editing for Plant Resistance to Abiotic Factors: A Systematic Review
	Nascimento, Fernanda dos Santos; Rocha, Anelita de Jesus; da Silva Soares, Julianna Matos; Mascarenhas, Marcelly Santana; Ferreira, Mileide dos Santos; Morais Lino, Lucymeire Souza; de Souza Ramos, Andresa Priscila; Cardamone Diniz, Leandro Eugenio; de Oliveira Mendes, Tiago Antonio; Ferreira, Claudia Fortes; dos Santos-Serejo, Janay Almeida; Amorim, Edson Perito
发表日期	2023
EISSN	2223-7747
卷号	12 期号:2
英文摘要	Agricultural crops are exposed to various abiotic stresses, such as salinity, water deficits, temperature extremes, floods, radiation, and metal toxicity. To overcome these challenges, breeding programs seek to improve methods and techniques. Gene editing by Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR/Cas-is a versatile tool for editing in all layers of the central dogma with focus on the development of cultivars of plants resistant or tolerant to multiple biotic or abiotic stresses. This systematic review (SR) brings new contributions to the study of the use of CRISPR/Cas in gene editing for tolerance to abiotic stress in plants. Articles deposited in different electronic databases, using a search string and predefined inclusion and exclusion criteria, were evaluated. This SR demonstrates that the CRISPR/Cas system has been applied to several plant species to promote tolerance to the main abiotic stresses. Among the most studied crops are rice and Arabidopsis thaliana, an important staple food for the population, and a model plant in genetics/biotechnology, respectively, and more recently tomato, whose number of studies has increased since 2021. Most studies were conducted in Asia, specifically in China. The Cas9 enzyme is used in most articles, and only Cas12a is used as an additional gene editing tool in plants. Ribonucleoproteins (RNPs) have emerged as a DNA-free strategy for genome editing without exogenous DNA. This SR also identifies several genes edited by CRISPR/Cas, and it also shows that plant responses to stress factors are mediated by many complex-signaling pathways. In addition, the quality of the articles included in this SR was validated by a risk of bias analysis. The information gathered in this SR helps to understand the current state of CRISPR/Cas in the editing of genes and noncoding sequences, which plays a key role in the regulation of various biological processes and the tolerance to multiple abiotic stresses, with potential for use in plant genetic improvement programs.
英文关键词	CRISPR; Cas9; Cas12a; genome editing; abiotic stresses; genetic improvement; tolerance; state of the art
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000918785000001
来源期刊	PLANTS-BASEL
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/281585
作者单位	Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA); Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA); Universidade Federal de Vicosa
推荐引用方式 GB/T 7714	Nascimento, Fernanda dos Santos,Rocha, Anelita de Jesus,da Silva Soares, Julianna Matos,et al. Gene Editing for Plant Resistance to Abiotic Factors: A Systematic Review[J],2023,12(2).
APA	Nascimento, Fernanda dos Santos.,Rocha, Anelita de Jesus.,da Silva Soares, Julianna Matos.,Mascarenhas, Marcelly Santana.,Ferreira, Mileide dos Santos.,...&Amorim, Edson Perito.(2023).Gene Editing for Plant Resistance to Abiotic Factors: A Systematic Review.PLANTS-BASEL,12(2).
MLA	Nascimento, Fernanda dos Santos,et al."Gene Editing for Plant Resistance to Abiotic Factors: A Systematic Review".PLANTS-BASEL 12.2(2023).