气候变化领域集成服务门户(CCPortal): Nitrogen Removal Characteristics and Constraints of an Alphaproteobacteria with Potential for High Nitrogen Content Heterotrophic Nitrification-Aerobic Denitrification

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DOI	10.3390/microorganisms10020235
	Nitrogen Removal Characteristics and Constraints of an Alphaproteobacteria with Potential for High Nitrogen Content Heterotrophic Nitrification-Aerobic Denitrification
	Zhang, Nan; Zhang, Yiting; Bohu, Tsing; Wu, Shanghua; Bai, Zhihui; Zhuang, Xuliang
通讯作者	Zhuang, XL (通讯作者)
发表日期	2022
EISSN	2076-2607
卷号	10 期号:2
英文摘要	The discovery of heterotrophic nitrification-aerobic denitrification (HN-AD) microorganisms has opened a new window for wastewater treatment. The underlying mechanism of HN-AD, however, is not fully understood because of the phylogenetic diversity of HN-AD microbes. The isolation and characterization of new HN-AD microorganisms are encouraging for furthering the understanding of this process. In this study, we found an Alphaproteobacteria isolate W30 from a historically polluted river in China through an HN-AD microbes screening process, which we identified as Pannonibacter sp. A potential HN-AD pathway for W30 was proposed based on N conversion analyses and the successful amplification of the entire denitrification gene series. The isolate exhibited high efficiency of aerobic inorganic nitrogen transformation, which accounted for 97.11% of NH4+-N, 100% of NO3--N, and 99.98% of NO2--N removal with a maximum linear rate of 10.21 mg/L/h, 10.46 mg/L/h, and 10.77 mg/L/h, respectively. Assimilation rather than denitrification was the main mechanism for the environmental nitrogen depletion mediated by W30. The effect of environmental constraints on aerobic NO3--N removal were characterized, following a membrane bioreactor effluent test under an oxic condition. Compared to known Alphaproteobacterial HN-AD microbes, we showed that Pannonibacter sp. W30 could deplete nitrogen with no NO2--N or NO3--N accumulation in the HN-AD process. Therefore, the application of Pannonibacter sp. W30 has the potential for developing a felicitous HN-AD technology to treat N-laden wastewater at the full-scale level.
关键词	AMMONIUM REMOVAL DENITRIFYING BACTERIUM STRAIN NITRITE NITRATE OXYGEN NIRK
英文关键词	aerobic; denitrification; heterotrophic; nitrification; Pannonibacter; wastewater
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000762678400001
来源期刊	MICROORGANISMS
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/260743
推荐引用方式 GB/T 7714	Zhang, Nan,Zhang, Yiting,Bohu, Tsing,et al. Nitrogen Removal Characteristics and Constraints of an Alphaproteobacteria with Potential for High Nitrogen Content Heterotrophic Nitrification-Aerobic Denitrification[J]. 中国科学院青藏高原研究所,2022,10(2).
APA	Zhang, Nan,Zhang, Yiting,Bohu, Tsing,Wu, Shanghua,Bai, Zhihui,&Zhuang, Xuliang.(2022).Nitrogen Removal Characteristics and Constraints of an Alphaproteobacteria with Potential for High Nitrogen Content Heterotrophic Nitrification-Aerobic Denitrification.MICROORGANISMS,10(2).
MLA	Zhang, Nan,et al."Nitrogen Removal Characteristics and Constraints of an Alphaproteobacteria with Potential for High Nitrogen Content Heterotrophic Nitrification-Aerobic Denitrification".MICROORGANISMS 10.2(2022).