气候变化领域集成服务门户(CCPortal): Screening and characterization of potent poly glutamic acid producing Bacillus sp. isolated from Kinema, water and soil samples

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DOI	10.1016/j.heliyon.2021.e07715
	Screening and characterization of potent poly glutamic acid producing Bacillus sp. isolated from Kinema, water and soil samples
	Thapa, Punam; Thapa, Alina; Khadka, Sujan; Sapkota, Sanjeep; Panta, Om Prakash; Sharma, Suprina; Karki, Tika Bahadur; Poudel, Pramod
通讯作者	Poudel, P (通讯作者)
发表日期	2021
EISSN	2405-8440
卷号	7 期号:8
英文摘要	Microbially produced gamma poly glutamic acid (gamma-PGA) is a commercially important biopolymer with many applications in foods and various other substances and are abundantly used in different parts of the world. With an aim to study the potent gamma-PGA producing Bacillus species, a total of 47 different samples (Kinema, soil, and water) were randomly collected from different locations across the country, and Bacillus sp. were selectively isolated, screened, and characterized by performing physiological, biochemical, morphological, and 16S rRNA gene sequencing. The microbial production of gamma-PGA was assayed with the selected isolates on the PGA medium and the metabolite obtained was recovered by ethanol precipitation method and further characterized by thin-layer chromatography (TLC). Thermotolerance (25-60 degrees C), pH tolerance (4-9), and NaCl tolerance (1-9%) tests were performed to optimize the bacterial growth and gamma-PGA production and its viscosity were measured by Ostwald's viscometer. Out of 145 randomly selected colonies, 63 isolates were Gram-positive, rods, and endospore producers and were presumptively confirmed as genus Bacillus. Higher growth of gamma-PGA producers were reported in 22 isolates and was found at optimum conditions such as temperature (30-37 C), pH (6.5-7), incubation time(3 days), and NaCl concentration (3%) and gamma-PGA thus produced was further verified by TLC with the retention factor (RF) value 0.27. The potent isolates were closely similar to Bacillus subtilis subsp. stercoris, Bacillus cereus, Bacillus paranthracis, and Bacillus licheniformis etc. Based on the findings of the study, B. licheniformis is the most potent gamma-PGA producing Bacillus sp. which can further be used for the commercial production of gamma-PGA. To the best of our knowledge, there is yet no published research from Nepal showing the production of the gamma-PGA although microbially produced gamma-PGA are the major constituents in some popular foods in particular communities of the country.
关键词	GAMMA-POLYGLUTAMIC ACID POLY(GAMMA-GLUTAMIC ACID)SUBTILIS NATTO LICHENIFORMIS
英文关键词	gamma-PGA; Bacillus species; Optimization; 16S rRNA gene
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000691788700016
来源期刊	HELIYON
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/260428
推荐引用方式 GB/T 7714	Thapa, Punam,Thapa, Alina,Khadka, Sujan,et al. Screening and characterization of potent poly glutamic acid producing Bacillus sp. isolated from Kinema, water and soil samples[J]. 中国科学院青藏高原研究所,2021,7(8).
APA	Thapa, Punam.,Thapa, Alina.,Khadka, Sujan.,Sapkota, Sanjeep.,Panta, Om Prakash.,...&Poudel, Pramod.(2021).Screening and characterization of potent poly glutamic acid producing Bacillus sp. isolated from Kinema, water and soil samples.HELIYON,7(8).
MLA	Thapa, Punam,et al."Screening and characterization of potent poly glutamic acid producing Bacillus sp. isolated from Kinema, water and soil samples".HELIYON 7.8(2021).