气候变化领域集成服务门户(CCPortal): Horizontal and vertical gene transfer drive sediment antibiotic resistome in an urban lagoon system

CCPortal

DOI	10.1016/j.jes.2020.09.004
	Horizontal and vertical gene transfer drive sediment antibiotic resistome in an urban lagoon system
	Wang, Hongjie; Hou, Liyuan; Liu, Yongqin; Liu, Keshao; Zhang, Lanping; Huang, Fuyi; Wang, Lin; Rashid, Azhar; Hu, Anyi; Yu, Changping
通讯作者	Hu, AY ; Yu, CP (通讯作者)
发表日期	2021
ISSN	1001-0742
EISSN	1878-7320
起始页码	11
结束页码	23
卷号	102
英文摘要	Rapid urbanization has resulted in pervasive occurrence of antibiotic resistance genes (ARGs) in urban aquatic ecosystems. However, limited information is available concerning the ARG profiles and the forces responsible for their assembly in urban landscape lagoon systems. Here, we employed high-throughput quantitative PCR (HT-qPCR) to characterize the spatial variations of ARGs in surface and core sediments of Yundang Lagoon, China. The results indicated that the average richness and absolute abundance of ARGs were 11 and 53 times higher in the lagoon sediments as compared to pristine reference Tibetan lake sediments, highlighting the role of anthropogenic activities in ARG pollution. Co-occurrence network analysis indicated that various anaerobic prokaryotic genera belonging to Alpha, Deltaproteobacteria, Bacteroidetes, Euryarchaeota, Firmicutes and Synergistetes were the potential hosts of ARGs. The partial least squares-path modeling (PLS-PM) analysis revealed positive and negative indirect effects of physicochemical factors and heavy metals on the lagoon ARG profiles, via biotic factors, respectively. The horizontal (mediated by mobile genetic elements) and vertical (mediated by prokaryotic communities) gene transfer may directly contribute the most to drive the abundance and composition of ARGs, respectively. Furthermore, the neutral community model demonstrated that the assembly of sediment ARG communities was jointly governed by deterministic and stochastic processes. Overall, this study provides novel insights into the diversity and distribution of ARGs in the benthic habitat of urban lagoon systems and underlying mechanisms for the spread and proliferation of ARGs. (C) 2020 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
关键词	RESISTANCE GENES BACTERIAL COMMUNITIES MICROBIAL COMMUNITIES HEAVY-METALS IDENTIFICATION ESTUARY ARGS LAKE MICROORGANISMS PROPAGATION
英文关键词	Urban landscape lagoon; Antibiotic resistance genes; Heavy metals; Horizontal and vertical genetransfer; Assembly mechanisms; High-throughput quantitative PCR
语种	英语
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000621513900002
来源期刊	JOURNAL OF ENVIRONMENTAL SCIENCES
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/260374
推荐引用方式 GB/T 7714	Wang, Hongjie,Hou, Liyuan,Liu, Yongqin,et al. Horizontal and vertical gene transfer drive sediment antibiotic resistome in an urban lagoon system[J]. 中国科学院青藏高原研究所,2021,102.
APA	Wang, Hongjie.,Hou, Liyuan.,Liu, Yongqin.,Liu, Keshao.,Zhang, Lanping.,...&Yu, Changping.(2021).Horizontal and vertical gene transfer drive sediment antibiotic resistome in an urban lagoon system.JOURNAL OF ENVIRONMENTAL SCIENCES,102.
MLA	Wang, Hongjie,et al."Horizontal and vertical gene transfer drive sediment antibiotic resistome in an urban lagoon system".JOURNAL OF ENVIRONMENTAL SCIENCES 102(2021).