气候变化领域集成服务门户(CCPortal): Functional Characterization of the Stipa purpurea P5CS Gene under Drought Stress Conditions

CCPortal

DOI	10.3390/ijms22179599
	Functional Characterization of the Stipa purpurea P5CS Gene under Drought Stress Conditions
	Yang, Danni; Ni, Ruize; Yang, Shihai; Pu, Yanan; Qian, Min; Yang, Yunqiang; Yang, Yongping
通讯作者	Yang, YQ ; Yang, YP (通讯作者)
发表日期	2021
EISSN	1422-0067
卷号	22 期号:17
英文摘要	Free proline has multiple functions in plant cells, such as regulating osmotic potential and protecting both proteins and cell membranes. The expression of Delta 1-Pyrroline-5-carboxylate synthase (P5CS), a key enzyme in the proline biosynthetic pathway, increases under drought, salt and cold stress conditions, causing plant cells to accumulate large amounts of proline. In this study, we cloned and identified the P5CS gene from Stipa purpurea, which has a full-length of 2196 bp and encodes 731 amino acids. A subcellular localization analysis indicated that SpP5CS localized to the cytoplasm. The ectopic overexpression of SpP5CS in Arabidopsis thaliana resulted in higher proline contents, longer roots, higher survival rates and less membrane damage under drought stress conditions compared with wild-type controls. SpP5CS-overexpressing A. thaliana was more resistant to drought stress than the wild type, whereas the deletion mutant sp5cs was less resistant to drought stress. Thus, SpP5CS may be a potential candidate target gene for increasing plant resistance to drought stress.
关键词	INDUCED PROLINE ACCUMULATION PLAY DISTINCT ROLES 1ST 2 STEPS DELTA(1)-PYRROLINE-5-CARBOXYLATE SYNTHETASE SALT STRESS CONFERS TOLERANCE EXPRESSION BIOSYNTHESIS L.DEGRADATION
英文关键词	Stipa purpurea; P5CS; proline; drought tolerance
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000694308500001
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/260239
推荐引用方式 GB/T 7714	Yang, Danni,Ni, Ruize,Yang, Shihai,et al. Functional Characterization of the Stipa purpurea P5CS Gene under Drought Stress Conditions[J]. 中国科学院青藏高原研究所,2021,22(17).
APA	Yang, Danni.,Ni, Ruize.,Yang, Shihai.,Pu, Yanan.,Qian, Min.,...&Yang, Yongping.(2021).Functional Characterization of the Stipa purpurea P5CS Gene under Drought Stress Conditions.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(17).
MLA	Yang, Danni,et al."Functional Characterization of the Stipa purpurea P5CS Gene under Drought Stress Conditions".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.17(2021).