气候变化领域集成服务门户(CCPortal): Automated flow cytometry as a flexible tool for comparing disinfection characteristics of indigenous bacterial communities and pure cultures

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DOI	10.1016/j.ecoenv.2021.112799
	Automated flow cytometry as a flexible tool for comparing disinfection characteristics of indigenous bacterial communities and pure cultures
	Mao, Guannan; Wang, Yingying; Hammes, Frederik
通讯作者	Wang, YY (通讯作者)
发表日期	2021
ISSN	0147-6513
EISSN	1090-2414
卷号	225
英文摘要	Bacterial inactivation efficiency of chlorine varies with organisms and environmental conditions. The comparability of different samples/studies, especially comparing indigenous bacterial communities with pure cultures, is impeded by inconsistent experimental conditions and analytical methods used in various studies. We tested a novel 96-well plate FCM experimental and automated analytical approach, where bacterial communities and pure cultures were suspended in the same natural water matrix prior to chlorination directly in the plate. We demonstrated the ability to rapidly monitor the efficiency of 32 different combinations of chlorine concentration and time (i.e. chlorine exposure) on bacterial pure cultures and indigenous aquatic communities, which enabled correct comparison of the data from different samples under the exact same experimental conditions. In this study, the 96-well plate automated FCM approach enabled large sets (896) of independent chlorination experiments to be carried out in a short time period. To our knowledge, this is the largest dataset of chlorination experiments which consumed least time (within 18 h after sampling) until now. Staining with SYBR Green I (SG) and SG combined with propidium iodide (SGPI) was used to assess cellular damage during chlorination. The results showed that with the same chlorine exposure, a higher chlorine concentration with a shorter contact time is favorable for inactivation of bacteria. Our research provides a promising framework to compare disinfection characteristics of various microorganism and can be further developed to diagnose effect of antimicrobial products.
关键词	DRINKING-WATER WASTE-WATER ESCHERICHIA-COLI CELL COUNTS CHLORINE RESISTANCE VIABILITY INACTIVATION ENUMERATION EFFICIENCY
英文关键词	Chlorination; Flow cytometry; Automation; Bacteria; Viability
语种	英语
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
WOS类目	Environmental Sciences ; Toxicology
WOS记录号	WOS:000703487300001
来源期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/260225
推荐引用方式 GB/T 7714	Mao, Guannan,Wang, Yingying,Hammes, Frederik. Automated flow cytometry as a flexible tool for comparing disinfection characteristics of indigenous bacterial communities and pure cultures[J]. 中国科学院青藏高原研究所,2021,225.
APA	Mao, Guannan,Wang, Yingying,&Hammes, Frederik.(2021).Automated flow cytometry as a flexible tool for comparing disinfection characteristics of indigenous bacterial communities and pure cultures.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,225.
MLA	Mao, Guannan,et al."Automated flow cytometry as a flexible tool for comparing disinfection characteristics of indigenous bacterial communities and pure cultures".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 225(2021).