气候变化领域集成服务门户(CCPortal): Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity

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DOI	10.1007/s00284-020-01976-7
	Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity
	Khadka, Sujan; Adhikari, Sanjib; Thapa, Alina; Panday, Raju; Adhikari, Manjila; Sapkota, Sanjeep; Regmi, Ramesh Sharma; Adhikari, Namita Paudel; Proshad, Ram; Koirala, Niranjan
通讯作者	Adhikari, S (通讯作者)
发表日期	2020
ISSN	0343-8651
EISSN	1432-0991
起始页码	1558
结束页码	1568
卷号	77 期号:8
英文摘要	The current study was designed to isolate, identify and characterize a Bacillus sp. capable of producing protease and exhibiting antifungal activity. A highly potent bacterium capable of producing protease abundantly was isolated from the soil collected from the waste pit near Microbiology Laboratory of Birendra Multiple Campus, Bharatpur and later on identified as Lysinibacillus fusiformis strain SK on the basis of morphological, physiological, biochemical and 16S rDNA gene sequencing techniques. The strain SK showed 98.36% similarity with L. fusiformis strain NBRC 15717. Using R-programming statistical analysis tool, the optimum incubation time for the highest average protease production (APP) (47.2 U/mL) was found to be 22 h at 50 degrees C and both incubation time and temperature showed a significant impact on the production of protease (P < 0.01). The maximum average relative protease activity (ARPA) was observed at pH 7.8 and 48 degrees C, whereas the least ARPA was observed in the presence of 80 g/L NaCl and 10 g/L HgCl2 (P < 0.01). The newly isolated bacterial strain also exhibited strong antifungal activity against aflatoxigenic Aspergillus flavus and Aspergillus parasiticus suggesting that it can be a potential candidate for protease production and activity over a wider range of temperature and pH as well as for synthesizing effective antifungal compounds.
关键词	ASPERGILLUS-FLAVUS GENOMICS ALKALINE PROTEASE SP NOV.BACILLUS ITURIN OCHRATOXIN MYCOTOXINS
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000523426600002
来源期刊	CURRENT MICROBIOLOGY
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/260069
推荐引用方式 GB/T 7714	Khadka, Sujan,Adhikari, Sanjib,Thapa, Alina,et al. Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity[J]. 中国科学院青藏高原研究所,2020,77(8).
APA	Khadka, Sujan.,Adhikari, Sanjib.,Thapa, Alina.,Panday, Raju.,Adhikari, Manjila.,...&Koirala, Niranjan.(2020).Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity.CURRENT MICROBIOLOGY,77(8).
MLA	Khadka, Sujan,et al."Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity".CURRENT MICROBIOLOGY 77.8(2020).