气候变化领域集成服务门户(CCPortal): Methane production in the oxygenated water column of a perennially ice-covered Antarctic lake

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DOI	10.1002/lno.11257
	Methane production in the oxygenated water column of a perennially ice-covered Antarctic lake
	Li, Wei; Dore, John E.; Steigmeyer, August J.; Cho, Yong-Joon; Kim, Ok-Sun; Liu, Yongqin; Morgan-Kiss, Rachael M.; Skidmore, Mark L.; Priscu, John C.
通讯作者	Priscu, JC (通讯作者)
发表日期	2020
ISSN	0024-3590
EISSN	1939-5590
起始页码	143
结束页码	156
卷号	65 期号:1
英文摘要	Aerobic methane production in aquatic ecosystems impacts the global atmospheric budget of methane, but the extent, mechanism, and taxa responsible for producing this greenhouse gas are not fully understood. Lake Bonney (LB), a perennially ice-covered Antarctic lake, has cold hypersaline waters underlying an oxygenated freshwater layer. We present temporal methane concentration profiles in LB indicating methane production in the oxygenated (> 200% air saturation) water. Experiments amended with methylphosphonate (MPn) yielded methane generation, suggesting in situ methanogenesis via the carbon-phosphorus (C-P) lyase pathway. Enrichment cultures from the lake were used to isolate five bacterial strains capable of generating methane when supplied with MPn as the sole P source. Based on 16S rRNA gene sequencing, the isolates belong to the Proteobacteria (closely related to Marinomonas, Hoeflea, and Marinobacter genera) and Bacteroidetes (Algoriphagus genus). 16S rRNA metagenomic sequencing confirms the presence of these taxa in LB. None of the isolated species were reported to be capable to produce methane. In addition, orthologs of the phosphoenolpyruvate mutase gene (PepM) and methylphosphonate synthase (MPnS), enzymes involved in phosphonate and MPn biosynthesis, were widely spread in the LB shotgun metagenomic libraries; genes related to C-P lyase pathways (phn gene clusters) were also abundant. 16S rRNA and mcrA genes of anaerobic methanogens were absent in both 16S rRNA and metagenomics libraries. These data reveal that in situ aerobic biological methane production is likely a significant source of methane in LB.
关键词	MCMURDO DRY VALLEYS ATMOSPHERIC METHANE AEROBIC PRODUCTION TAYLOR GLACIER BLOOD FALLS SP-NOV.PHOSPHORUS DIVERSITY BACTERIAL METHYLPHOSPHONATE
语种	英语
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000476977600001
来源期刊	LIMNOLOGY AND OCEANOGRAPHY
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/259942
推荐引用方式 GB/T 7714	Li, Wei,Dore, John E.,Steigmeyer, August J.,et al. Methane production in the oxygenated water column of a perennially ice-covered Antarctic lake[J]. 中国科学院青藏高原研究所,2020,65(1).
APA	Li, Wei.,Dore, John E..,Steigmeyer, August J..,Cho, Yong-Joon.,Kim, Ok-Sun.,...&Priscu, John C..(2020).Methane production in the oxygenated water column of a perennially ice-covered Antarctic lake.LIMNOLOGY AND OCEANOGRAPHY,65(1).
MLA	Li, Wei,et al."Methane production in the oxygenated water column of a perennially ice-covered Antarctic lake".LIMNOLOGY AND OCEANOGRAPHY 65.1(2020).