气候变化领域集成服务门户(CCPortal): Responses of ammonia-oxidizing archaea and bacteria to nitrogen and phosphorus amendments in an alpine steppe

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DOI	10.1111/ejss.12911
	Responses of ammonia-oxidizing archaea and bacteria to nitrogen and phosphorus amendments in an alpine steppe
	Dong, Junfu; Che, Rongxiao; Jia, Shugang; Wang, Fang; Zhang, Biao; Cui, Xiaoyong; Wang, Shiping; Wang, Shuping
通讯作者	Wang, SP (通讯作者)
发表日期	2020
ISSN	1351-0754
EISSN	1365-2389
起始页码	940
结束页码	954
卷号	71 期号:5
英文摘要	With the increasing demands of livestock production, grasslands are under pressure from over-intensified grazing. Phosphorus (P) and nitrogen (N) fertilization is widely employed to meet the nutrient demands of heavy grazing. Although soil ammonia-oxidizers play a critical role in determining N dynamics after fertilizer application, their responses to fertilization are still not well understood in steppe grassland systems. Here, the individual and combined effects of N (0, 7.5 and 15 g N m(-2) year(-1)) and P (0, 3.27, 6.55 and 13.09 g P m(-2) year(-1)) applications on soil ammonia-oxidizers were explored in the Tibetan alpine steppe. Ammonia-oxidizing archaea (AOA) and bacteria (AOB) abundance and community composition were examined using qPCR, terminal restriction fragment length polymorphism and clone libraries. Results showed that AOB diversity was significantly increased by N fertilization and decreased by P fertilization. The abundance of AOB was significantly increased by N fertilization and its interactive effects with P application. In contrast, AOA community diversity and abundance remained unaffected by either N or P application. The AOB abundance and diversity were affected by the direct effects of N fertilization, as well as its indirect effects of N application through available N and ammonium (NH4+), and further analysis showed that NH4+ and pH were the main factors. The changes to the ammonia-oxidizing community altered the total N content of plants and the N:P ratio of Gramineae. Plant P properties were influenced by P addition, which also interacted with soil pH and available N to indirectly affect AOB. Overall, AOB exhibited stronger responses to N and P fertilization of alpine steppe grassland than AOA, and appear to play a critical role in plant nutrient absorption under fertilization management. Highlights Effects of N and P application on AOA and AOB communities were evaluated. AOB, not AOA, responded to N or P, and their interaction, and NH4+ and pH were regulators of AOB. N application affected AOB community, resulting in changes to plant nutrient absorption. P addition affected plant tissue stoichiometry, and thus affected AOB community.
关键词	COMMUNITY STRUCTURE SOIL-NITROGEN TEMPERATE STEPPE ORGANIC NITROGEN TIBETAN PLATEAU GRASSLAND SOIL ABUNDANCE NITRIFICATION FERTILIZATION DIVERSITY
英文关键词	ammonia oxidizing bacteria; archaea; N and P fertilization; Qinghai-Tibetan grassland degradation
语种	英语
WOS研究方向	Agriculture
WOS类目	Soil Science
WOS记录号	WOS:000504378100001
来源期刊	EUROPEAN JOURNAL OF SOIL SCIENCE
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/259925
推荐引用方式 GB/T 7714	Dong, Junfu,Che, Rongxiao,Jia, Shugang,et al. Responses of ammonia-oxidizing archaea and bacteria to nitrogen and phosphorus amendments in an alpine steppe[J]. 中国科学院青藏高原研究所,2020,71(5).
APA	Dong, Junfu.,Che, Rongxiao.,Jia, Shugang.,Wang, Fang.,Zhang, Biao.,...&Wang, Shuping.(2020).Responses of ammonia-oxidizing archaea and bacteria to nitrogen and phosphorus amendments in an alpine steppe.EUROPEAN JOURNAL OF SOIL SCIENCE,71(5).
MLA	Dong, Junfu,et al."Responses of ammonia-oxidizing archaea and bacteria to nitrogen and phosphorus amendments in an alpine steppe".EUROPEAN JOURNAL OF SOIL SCIENCE 71.5(2020).