气候变化领域集成服务门户(CCPortal): Abundance, diversity and mobility potential of antibiotic resistance genes in pristine Tibetan Plateau soil as revealed by soil metagenomics

CCPortal

DOI	10.1093/femsec/fiaa172
	Abundance, diversity and mobility potential of antibiotic resistance genes in pristine Tibetan Plateau soil as revealed by soil metagenomics
	Li, Bo; Chen, Zeng; Zhang, Fan; Liu, Yongqin; Yan, Tao
通讯作者	Yan, T (通讯作者)
发表日期	2020
ISSN	0168-6496
EISSN	1574-6941
卷号	96 期号:10
英文摘要	Widespread occurrence of antibiotic resistance genes (ARGs) has become an important clinical issue. Studying ARGs in pristine soil environments can help to better understand the intrinsic soil resistome. In this study, 10 soil samples were collected from a high elevation and relatively pristine Tibetan area, and metagenomic sequencing and bioinformatic analyses were conducted to investigate the microbial diversity, the abundance and diversity of ARGs and the mobility potential of ARGs as indicated by different mobile genetic elements (MGEs). A total of 48 ARG types with a relative abundance of 0.05-0.28 copies of ARG/copy of 16S rRNA genes were detected in Tibetan soil samples. The observed ARGs were mainly associated with antibiotics that included glycopeptide and rifamycin; the most abundant ARGs were vanRO and vanSO. Low abundance of MGEs and potentially plasmid-related ARGs indicated a low horizontal gene transfer risk of ARGs in the pristine soil. Pearson correlation and redundancy analyses showed that temperature and total organic carbon were the major environmental factors controlling both microbial diversity and ARG abundance and diversity.
关键词	WATER TREATMENT PLANTS MICROBIAL COMMUNITY BACTERIAL PATHOGENS ALPINE GRASSLANDS BETA-LACTAMASES RESISTOME ARGS IDENTIFICATION ENVIRONMENTS PERMAFROST
英文关键词	Tibetan Plateau; metagenomic sequencing; antibiotic resistance genes; mobile genetic elements
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000581018800006
来源期刊	FEMS MICROBIOLOGY ECOLOGY
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/259911
推荐引用方式 GB/T 7714	Li, Bo,Chen, Zeng,Zhang, Fan,et al. Abundance, diversity and mobility potential of antibiotic resistance genes in pristine Tibetan Plateau soil as revealed by soil metagenomics[J]. 中国科学院青藏高原研究所,2020,96(10).
APA	Li, Bo,Chen, Zeng,Zhang, Fan,Liu, Yongqin,&Yan, Tao.(2020).Abundance, diversity and mobility potential of antibiotic resistance genes in pristine Tibetan Plateau soil as revealed by soil metagenomics.FEMS MICROBIOLOGY ECOLOGY,96(10).
MLA	Li, Bo,et al."Abundance, diversity and mobility potential of antibiotic resistance genes in pristine Tibetan Plateau soil as revealed by soil metagenomics".FEMS MICROBIOLOGY ECOLOGY 96.10(2020).