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DOI	10.3389/fchem.2020.00197
	Potashchelins, a Suite of Lipid Siderophores Bearing Both L-threo and L-erythro Beta-Hydroxyaspartic Acids, Acquired From the Potash-Salt-Ore-Derived Extremophile Halomonas sp. MG34
	Li, Yihong; Liu, Li; Zhang, Gengxin; He, Ning; Guo, Wenqiang; Hong, Bin; Xie, Yunying
通讯作者	Hong, B ; Xie, YY (通讯作者)
发表日期	2020
ISSN	2296-2646
卷号	8
英文摘要	Four new lipid siderophores bearing both L-threo- and L-erythro-beta-hydroxyaspartic acids, potashchelins A-D (1-4), were isolated from the potash-salt-ore-derived extremophile Halomonas sp. MG34. The planar structures of 1-4 were elucidated on the basis of extensive 1D and 2D NMR studies and MS/MS data. Potashchelins 1-4 contain a hydrophilic nonapeptide headgroup sequentially consisting of beta-hydroxyaspartic acid, serine, glycine, serine, serine, beta-hydroxyaspartic acid, threonine, serine, and cyclic N(delta)-hydroxy-ornithine, which is appended by one of a series of fatty acids ranging from dodecanoic acid to tetradecanoic acid. The absolute configurations of the amino acids of potashchelins 1-4 were determined by C-3 and advanced Marfey's reaction, partial hydrolysis, and bioinformatics analysis, which revealed that potashchelins 1-4 bear both L-threo- and L-erythro-beta-hydroxyaspartic acid. Phylogenetic analysis showed that the stand-alone beta-hydroxylase, PtcA, and the fused domain with beta-hydroxylase activity in PtcB are expected to be responsible for the formation of L-erythro and L-threo diastereomers, respectively. Additionally, utilizing a comparative genomics approach, we revealed an evolutionary mechanism for lipid siderophores in Halomonas involving horizontal transfer. Bioassays showed that potashchelin A and D had weak antibacterial activity against B. subtilis CPCC 100029 with an MIC value of 64 mu g/mL.
关键词	GENOMICS-DRIVEN DISCOVERY CONSTITUENT AMINO-ACIDS AMPHIPHILIC SIDEROPHORES STRUCTURAL-CHARACTERIZATION ABSOLUTE-CONFIGURATION MARFEYS METHOD BIOSYNTHESIS SYRINGOMYCIN DOMAIN LC/MS
英文关键词	potashchelins; lipid siderophore; L-threo-beta-hydroxyaspartic acid; L-erythro-beta-hydroxyaspartic acid; Halomonas; beta-hydroxylases
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000523446800001
来源期刊	FRONTIERS IN CHEMISTRY
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/259750
推荐引用方式 GB/T 7714	Li, Yihong,Liu, Li,Zhang, Gengxin,et al. Potashchelins, a Suite of Lipid Siderophores Bearing Both L-threo and L-erythro Beta-Hydroxyaspartic Acids, Acquired From the Potash-Salt-Ore-Derived Extremophile Halomonas sp. MG34[J]. 中国科学院青藏高原研究所,2020,8.
APA	Li, Yihong.,Liu, Li.,Zhang, Gengxin.,He, Ning.,Guo, Wenqiang.,...&Xie, Yunying.(2020).Potashchelins, a Suite of Lipid Siderophores Bearing Both L-threo and L-erythro Beta-Hydroxyaspartic Acids, Acquired From the Potash-Salt-Ore-Derived Extremophile Halomonas sp. MG34.FRONTIERS IN CHEMISTRY,8.
MLA	Li, Yihong,et al."Potashchelins, a Suite of Lipid Siderophores Bearing Both L-threo and L-erythro Beta-Hydroxyaspartic Acids, Acquired From the Potash-Salt-Ore-Derived Extremophile Halomonas sp. MG34".FRONTIERS IN CHEMISTRY 8(2020).