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DOI	10.1007/s00253-020-10984-3
	Comparative genomic analysis reveals metabolic diversity of different Paenibacillus groups
	Huang, Wen-Cong; Hu, Yilun; Zhang, Gengxin; Li, Meng
通讯作者	Li, M (通讯作者)
发表日期	2020
ISSN	0175-7598
EISSN	1432-0614
起始页码	10133
结束页码	10143
卷号	104 期号:23
英文摘要	The genus Paenibacillus was originally recognized based on the 16S rRNA gene phylogeny. Recently, a standardized bacterial taxonomy approach based on a genome phylogeny has substantially revised the classification of Paenibacillus, dividing it into 23 genera. However, the metabolic differences among these groups remain undescribed. Here, genomes of 41 Paenibacillus strains comprising 25 species were sequenced, and a comparative genomic analysis was performed considering these and 187 publicly available Paenibacillus genomes to understand their phylogeny and metabolic differences. Phylogenetic analysis indicated that Paenibacillus clustered into 10 subgroups. Core genome and pan-genome analyses revealed similar functional categories among the different Paenibacillus subgroups; however, each group tended to harbor specific gene families. A large proportion of genes in the subgroups A, E, and G are related to carbohydrate metabolism. Among them, genes related to the glycoside hydrolase family were most abundant. Metabolic reconstruction of the newly sequenced genomes showed that the Embden-Meyerhof-Parnas pathway, pentose phosphate pathway, and citric acid cycle are central pathways of carbohydrate metabolism in Paenibacillus. Further, the genomes of the subgroups A and G lack genes involved in glyoxylate cycle and d-galacturonate degradation, respectively. The current study revealed the metabolic diversity of Paenibacillus subgroups assigned based on a genomic phylogeny and could inform the taxonomy of Paenibacillus.
关键词	SP NOV.D-GALACTURONATE IDENTIFICATION ALIGNMENT ALGORITHM ACID TREE
英文关键词	Paenibacillus; Phylogeny; Pan-genome; Metabolism; CAZymes
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000583129000004
来源期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/259718
推荐引用方式 GB/T 7714	Huang, Wen-Cong,Hu, Yilun,Zhang, Gengxin,et al. Comparative genomic analysis reveals metabolic diversity of different Paenibacillus groups[J]. 中国科学院青藏高原研究所,2020,104(23).
APA	Huang, Wen-Cong,Hu, Yilun,Zhang, Gengxin,&Li, Meng.(2020).Comparative genomic analysis reveals metabolic diversity of different Paenibacillus groups.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,104(23).
MLA	Huang, Wen-Cong,et al."Comparative genomic analysis reveals metabolic diversity of different Paenibacillus groups".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 104.23(2020).