气候变化领域集成服务门户(CCPortal): Total and active soil fungal community profiles were significantly altered by six years of warming but not by grazing

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DOI	10.1016/j.soilbio.2019.107611
	Total and active soil fungal community profiles were significantly altered by six years of warming but not by grazing
	Che, Rongxiao; Wang, Shiping; Wang, Yanfen; Xu, Zhihong; Wang, Weijin; Rui, Yichao; Wang, Fang; Hu, Jinming; Tao, Juan; Cui, Xiaoyong
通讯作者	Wang, YF ; Cui, XY (通讯作者)
发表日期	2019
ISSN	0038-0717
卷号	139
英文摘要	We examined the responses of total and active soil fungal communities to experimental warming, moderate grazing, and their combination. The six years of field experiments were established in a Tibetan alpine meadow. An infrared heating system was used to simulate warming, and the grazing was conducted by a combination of sheep grazing and clipping. Total and active soil fungal community profiles were determined using MiSeq sequencing of internal transcribed spacer (ITS) DNA and RNA, respectively. Fungal abundance and rDNA transcriptional activity were analyzed through real-time PCR. The results showed that the warming significantly changed the community structure of soil fungi, particularly the active populations. Specific changes in fungal community profiles under the warming scenario included an increase in the proportion of Dothideomycetes (a potential oligotrophic class) and a decrease in the proportion of active plant-symbiotic lineages (e.g., Glomerales). FUNGuild-based functional predictions suggested that warming significantly reduced the proportions of arbuscular mycorrhizal and active saprotrophic fungi. We compared our results to those of another investigation performed at the same location, and found that the ITS-FUNGuild approach was even more sensitive than the 18S rDNA-based method with respect to identifying arbuscular mycorrhizal fungal taxa. Additionally, the warming attenuated the interactions among fungal species, reduced the Chao 1 index of active fungal communities, increased the active fungal community dispersion, and tended to suppress the transcription of fungal rDNA. In contrast, no significant effects of grazing and warming-grazing interactions on soil fungal communities were observed. These findings indicate that global warming may weaken the capacity of soil fungi to decompose organic matter and to facilitate plant nutrient absorption, whereas fungi in Tibetan alpine meadow soils are insensitive to moderate grazing.
关键词	CLIMATE-CHANGE MICROBIAL COMMUNITY ORGANIC-CARBON RIBOSOMAL-RNA ALPINE MEADOW TEMPERATURE SENSITIVITY COOCCURRENCE PATTERNS BACTERIAL COMMUNITY SPECIES COMPOSITION ENZYME-ACTIVITY
英文关键词	Climate change; Livestock grazing; Tibetan plateau; Soil microbes; Active fungal communities
语种	英语
WOS研究方向	Agriculture
WOS类目	Soil Science
WOS记录号	WOS:000501654200001
来源期刊	SOIL BIOLOGY & BIOCHEMISTRY
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/259598
推荐引用方式 GB/T 7714	Che, Rongxiao,Wang, Shiping,Wang, Yanfen,et al. Total and active soil fungal community profiles were significantly altered by six years of warming but not by grazing[J]. 中国科学院青藏高原研究所,2019,139.
APA	Che, Rongxiao.,Wang, Shiping.,Wang, Yanfen.,Xu, Zhihong.,Wang, Weijin.,...&Cui, Xiaoyong.(2019).Total and active soil fungal community profiles were significantly altered by six years of warming but not by grazing.SOIL BIOLOGY & BIOCHEMISTRY,139.
MLA	Che, Rongxiao,et al."Total and active soil fungal community profiles were significantly altered by six years of warming but not by grazing".SOIL BIOLOGY & BIOCHEMISTRY 139(2019).