气候变化领域集成服务门户(CCPortal): Long-term warming rather than grazing significantly changed total and active soil procaryotic community structures

CCPortal

DOI	10.1016/j.geoderma.2017.12.005
	Long-term warming rather than grazing significantly changed total and active soil procaryotic community structures
	Che, Rongxiao; Deng, Yongcui; Wang, Weijin; Rui, Yichao; Zhang, Jing; Tahmasbian, Iman; Tang, Li; Wang, Shiping; Wang, Yanfen; Xu, Zhihong; Cui, Xiaoyong
通讯作者	Cui, XY (通讯作者)
发表日期	2018
ISSN	0016-7061
EISSN	1872-6259
起始页码	1
结束页码	10
卷号	316
英文摘要	There is a paucity of knowledge in understanding the effects of warming and grazing on soil microbes and their active counterparts, especially on the Tibetan Plateau which is extremely sensitive to global warming and human activities. A six-year field experiment was conducted to investigate the effects of asymmetric warming and moderate grazing on total and active soil microbes in a Tibetan Kobresia alpine meadow. Soil bacterial abundance and 16S rDNA transcriptional activity were determined using real-time PCR. Total and active soil procaryotic community structures were analyzed through MiSeq sequencing based on 16S rDNA and rRNA, respectively. The results showed that the soil procaryotic community was more sensitive to the warming than the grazing. The warming significantly decreased soil microbial respiration rates, 16S rDNA transcription activity, and dispersion of total procaryotic community structures, but significantly increased the alpha diversity of active procaryotes. Warming also significantly increased the relative abundance of oligotrophic microbes, whereas decreasing the copiotrophic lineage proportions. The functional profiles predicted from the total procaryotic community structures remained unaffected by warming. However, the rRNA-based predictions suggested that DNA replication, gene expression, signal transduction, and protein degradation were significantly suppressed under the warming. The grazing only significantly decreased the 16S rDNA transcription and total procaryotic richness. Overall, these findings suggest that warming can shift soil procaryotic community to a more oligotrophic and less active status, highlighting the importance of investigating active microbes to improve our understanding of ecosystem feedbacks to climate change and human activities.
关键词	16S RIBOSOMAL-RNA MICROBIAL COMMUNITY BACTERIAL COMMUNITY CLIMATE-CHANGE ALPINE MEADOW TEMPERATURE SENSITIVITY TIBETAN PLATEAU WATER-CONTENT RESPIRATION CARBON
英文关键词	Climate changes; Livestock grazing; Tibetan plateau; Soil microbes; Microbial activity
语种	英语
WOS研究方向	Agriculture
WOS类目	Soil Science
WOS记录号	WOS:000424179300001
来源期刊	GEODERMA
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/259333
推荐引用方式 GB/T 7714	Che, Rongxiao,Deng, Yongcui,Wang, Weijin,et al. Long-term warming rather than grazing significantly changed total and active soil procaryotic community structures[J]. 中国科学院青藏高原研究所,2018,316.
APA	Che, Rongxiao.,Deng, Yongcui.,Wang, Weijin.,Rui, Yichao.,Zhang, Jing.,...&Cui, Xiaoyong.(2018).Long-term warming rather than grazing significantly changed total and active soil procaryotic community structures.GEODERMA,316.
MLA	Che, Rongxiao,et al."Long-term warming rather than grazing significantly changed total and active soil procaryotic community structures".GEODERMA 316(2018).