气候变化领域集成服务门户(CCPortal): Sphingomonas montana sp nov., isolated from a soil sample from the Tanggula Mountain in the Qinghai Tibetan Plateau

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DOI	10.1007/s10482-017-0915-6
	Sphingomonas montana sp nov., isolated from a soil sample from the Tanggula Mountain in the Qinghai Tibetan Plateau
	Manandhar, Pooja; Zhang, Gengxin; Lama, Arun; Liu, Fei; Hu, Yilun
通讯作者	Zhang, GX (通讯作者)
发表日期	2017
ISSN	0003-6072
EISSN	1572-9699
起始页码	1659
结束页码	1668
卷号	110 期号:12
英文摘要	An orange pigmented, Gram-staining negative, aerobic, motile, rod-shaped bacterium isolated from a soil from the Tanggula Mountain, China was studied using a polyphasic approach. Based on 16S rRNA gene sequence similarity, strain W16RD(T) was found to be closely related to Sphingomonas prati DSM 103336(T) (99%), Sphingomonas fennica DSM 13665(T) (97.21%), followed by Sphingomonas laterariae DSM 25432(T) (96.44%), Sphingomonas haloaromaticamans CGMCC 1.10206 (T) (96.36%) and Sphingomonas formosensis DSM 24164(T) (96.06%). The strain was found to be catalase and oxidase positive and was found to grow optimally at temperatures of 20-25 A degrees C, pH 8 and tolerated NaCl concentration up to 1% (w/v). The major fatty acids identified were summed feature eight comprising C-18:1 omega 7c and/or C-18:1 omega 6c (39.2%), summed feature three comprising of C-16:1 omega 7c and/or C-16:1 omega 6c (36.7%) and C-16:0 (7.0%). The polar lipids detected were phosphatidylcholine, sphingoglycolipid, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidyldimethylethanolamine, phosphatidylmonomethylethanolamine, and three unidentified lipids. The strain possessed ubiquinone-10 (Q-10) as the predominant respiratory quinone. Along with other distinguishing characteristics, we also describe the draft genome of strain W16RD(T). The final assembled draft genome sequence is 3,722,743 bp with 3390 coding and 48 RNA (45 tRNA and 3 rRNA) genes. The DNA G+C content of the genomic DNA was determined to be 67%. The DNA-DNA relatedness value between the strain W16RD(T) and its closest phylogenetic relatives S. prati DSM 103336(T), S. fennica DSM 13665(T), S. laterariae DSM 25432(T), and S. haloaromaticamans CGMCC 1.10206(T) were 52.17, 47.60, 20.93 and 17.09% respectively. The strain W16RD(T) could be distinguished genotypically and phenotypically from the recognized species belonging to the genus Sphingomonas and thus represents a novel species, for which the name Sphingomonas montana sp. nov. is proposed. The type strain is W16RD(T) (=CGMCC 1.15646(T) = DSM 103337(T)).
关键词	EMENDED DESCRIPTION BACTERIUM AIR DNA
英文关键词	Sphingomonas montana sp nov; Soil; 16S rRNA gene; Whole genome
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000414779100013
来源期刊	ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/258989
推荐引用方式 GB/T 7714	Manandhar, Pooja,Zhang, Gengxin,Lama, Arun,et al. Sphingomonas montana sp nov., isolated from a soil sample from the Tanggula Mountain in the Qinghai Tibetan Plateau[J]. 中国科学院青藏高原研究所,2017,110(12).
APA	Manandhar, Pooja,Zhang, Gengxin,Lama, Arun,Liu, Fei,&Hu, Yilun.(2017).Sphingomonas montana sp nov., isolated from a soil sample from the Tanggula Mountain in the Qinghai Tibetan Plateau.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,110(12).
MLA	Manandhar, Pooja,et al."Sphingomonas montana sp nov., isolated from a soil sample from the Tanggula Mountain in the Qinghai Tibetan Plateau".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 110.12(2017).