气候变化领域集成服务门户(CCPortal): Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics

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DOI	10.3389/fmicb.2017.01406
	Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics
	Wang, Shaochen; Gao, Xia; Gao, Yuejiao; Li, Yanqing; Cao, Mingming; Xi, Zhenhua; Zhao, Lixing; Feng, Zhiyang
通讯作者	Feng, ZY (通讯作者)
发表日期	2017
ISSN	1664-302X
卷号	8
英文摘要	Soil microbiota represents one of the ancient evolutionary origins of antibiotic resistance and has been increasingly recognized as a potentially vast unstudied reservoir of resistance genes with possibilities to exchange with pathogens. Tetracycline resistance is one of the most abundant antibiotic resistances that may transfer among clinical and commensal microorganisms. To investigate tetracycline resistance genes from soil bacteria in different habitats, we performed functional analysis of three metagenomic libraries derived from soil samples collected from Yunnan, Sichuan, and Tibet, respectively, in China. We found efflux transporter genes form all the libraries, including 21 major facilitator superfamily efflux pump genes and one multidrug and toxic compound extrusion (MATE) transporter gene. Interestingly, we also identified two tetracycline destructase genes, belonging to a newly described family of tetracycline-inactivating enzymes that scarcely observed in clinical pathogens, from the Tibet library. The inactivation activity of the putative enzyme was confirmed in vitro by biochemical analysis. Our results indicated that efflux pumps distributed predominantly across habitats. Meanwhile, the mechanism of enzymatic inactivation for tetracycline resistance should not be neglected and merits further investigation.
关键词	ANTIBIOTIC-RESISTANCE RESISTOME DETERMINANT BACTERIA REVEALS POULTRY FOOD
英文关键词	functional metagenomics; soil; tetracycline resistance; efflux transporter; tetracycline destructase
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000406337800001
来源期刊	FRONTIERS IN MICROBIOLOGY
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/258955
推荐引用方式 GB/T 7714	Wang, Shaochen,Gao, Xia,Gao, Yuejiao,et al. Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics[J]. 中国科学院青藏高原研究所,2017,8.
APA	Wang, Shaochen.,Gao, Xia.,Gao, Yuejiao.,Li, Yanqing.,Cao, Mingming.,...&Feng, Zhiyang.(2017).Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics.FRONTIERS IN MICROBIOLOGY,8.
MLA	Wang, Shaochen,et al."Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics".FRONTIERS IN MICROBIOLOGY 8(2017).