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DOI	10.1007/s11430-015-5240-1
	Application of orthogonal design to the extraction and HPLC analysis of sedimentary pigments from lakes of the Tibetan Plateau
	Liang Jie; Li Dong; Wang MingDa; Zhang XinYu; Hou JuZhi
通讯作者	Hou, JZ (通讯作者)
发表日期	2016
ISSN	1674-7313
EISSN	1869-1897
起始页码	1195
结束页码	1205
卷号	59 期号:6
英文摘要	Sedimentary pigments are useful proxy indicators of phytoplankton biomass, community structure, primary productivity and human influence in lacustrine and oceanic ecosystems. Pigments are relatively labile due to their complex chemical structures, which makes the extraction and analysis of sedimentary pigments challenging. In addition, it is important to select appropriate methods to study sedimentary pigments in regions which lack previous investigations. In this study, we adopted the L-9(3(4)) orthogonal design to develop methods of extraction and HPLC (high performance liquid chromatography) analysis of sedimentary pigments at two lakes on the Tibetan Plateau: meromictic lake-Dagze Co and dimictic lake-Jiang Co. The orthogonal design comprises 9 combinations of various parameters for extraction and HPLC analysis. The results show that the type and volume of solvent are the most important factors for pigment extraction, and the mobile phase and column selection are the most important for HPLC analysis. For the study sites, we found that the best methods to extract sedimentary pigments are as follows: the use of a mixture of acetone:methanol:water (80:15:5, v:v:v) as the extraction solvent; solvent/ sample ratio of 10 mL/g; sonication for 30 s and standing extraction for 6 h. The best methods for HPLC analysis are as follows: Zorbax Eclipse plus C8 column with mobile phase A, methanol:acetonitrile:0.25 M aqueous pyridine (50:25:25, v:v:v) and mobile phase B, methanol: acetonitrile: acetone (20:60:20, v:v:v); pH of mobile phase A adjusted to 6 with acetic acid; and HPLC column temperature maintained at 40A degrees C. The study provides an experimental basis for future investigations of past changes in primary productivity and the response of lake ecosystems to climate change and human activities on the Tibetan Plateau.
关键词	PHYTOPLANKTON CHLOROPHYLLS CAROTENOIDS COLUMN CLASSIFICATION TEMPERATURE ABUNDANCE PROXY
英文关键词	Tibetan Plateau; Sedimentary pigments; Pigment extraction; Pigment analysis; Orthogonal design
语种	英语
WOS研究方向	Geology
WOS类目	Geosciences, Multidisciplinary
WOS记录号	WOS:000376675400007
来源期刊	SCIENCE CHINA-EARTH SCIENCES
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/258831
推荐引用方式 GB/T 7714	Liang Jie,Li Dong,Wang MingDa,et al. Application of orthogonal design to the extraction and HPLC analysis of sedimentary pigments from lakes of the Tibetan Plateau[J]. 中国科学院青藏高原研究所,2016,59(6).
APA	Liang Jie,Li Dong,Wang MingDa,Zhang XinYu,&Hou JuZhi.(2016).Application of orthogonal design to the extraction and HPLC analysis of sedimentary pigments from lakes of the Tibetan Plateau.SCIENCE CHINA-EARTH SCIENCES,59(6).
MLA	Liang Jie,et al."Application of orthogonal design to the extraction and HPLC analysis of sedimentary pigments from lakes of the Tibetan Plateau".SCIENCE CHINA-EARTH SCIENCES 59.6(2016).