气候变化领域集成服务门户(CCPortal): Sphingomonas prati sp nov., isolated from alpine meadow soil

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DOI	10.1099/ijsem.0.001346
	Sphingomonas prati sp nov., isolated from alpine meadow soil
	Manandhar, Pooja; Zhang, Gengxin; Hu, Yilun; Lama, Arun; Gao, Feng; Gu, Zhengquan
通讯作者	Zhang, GX (通讯作者)
发表日期	2016
ISSN	1466-5026
EISSN	1466-5034
起始页码	4269
结束页码	4275
卷号	66
英文摘要	A Gram-staining-negative, non-motile, orange-coloured and rod-shaped aerobic bacterium, designated W18RD(T), was isolated from the alpine meadow soil of the Tibetan plateau. Phylogenetic analysis based on 16S rRNA gene sequences positioned strain W18RD(T) as a representative of a novel species under the genus Sphingomonas which was most closely related to Sphingomonas fennica DSM 13665(T) with a sequence similarity level of 97.14 %. Meanwhile, it also had a high level of sequence similarity with Sphingomonas laterariae DSM 25432(T) (96.51 %), Sphingomonas haloaromaticamans CGMCC 1.10206(T) (96.43 %) and Sphingomonas formosensis DSM 24164(T) (96.26 %). The G+C content of the genomic DNA of the type strain W18RD(T) was 66.4mol%. DNA-DNA relatedness for the type strain W18RD(T) with respect to its closest phylogenetic relative Sphingomonas. fennica DSM 13665(T) was 21.54 +/- 1.2 %. Major cellular fatty acids in strain W18RD(T) were C-16 (:) (1)omega 7c and/or C-16 (:) (1)omega 6c (48.12 %), C-18 (:) (1)omega 7c and/or C-18 (:) (1)omega 6c (21.98 %) and C-14 (:) (0) 2-OH (14.93 %), with ubiquinone-10 (Q-10) as the predominant respiratory quinone. The polar lipid profile of the strain consisted of phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphatidylcholine, diphosphatidylglycerol and two unknown lipids. Based on the evidence from a combination of phenotypic, taxonomic and phylogenetic analyses, strain W18RD(T) represents a novel species of the genus Sphingomonas, for which the name Sphingomonas prati sp. nov. is proposed. The type strain is W18RD(T) (=CGMCC 1.15645(T)= DSM 103336(T)).
关键词	EMENDED DESCRIPTION DNA HYBRIDIZATION BACTERIUM NOVOSPHINGOBIUM SPHINGOPYXIS SPHINGOBIUM EMENDATION GENERA AIR
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000386871600080
来源期刊	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/258786
推荐引用方式 GB/T 7714	Manandhar, Pooja,Zhang, Gengxin,Hu, Yilun,et al. Sphingomonas prati sp nov., isolated from alpine meadow soil[J]. 中国科学院青藏高原研究所,2016,66.
APA	Manandhar, Pooja,Zhang, Gengxin,Hu, Yilun,Lama, Arun,Gao, Feng,&Gu, Zhengquan.(2016).Sphingomonas prati sp nov., isolated from alpine meadow soil.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,66.
MLA	Manandhar, Pooja,et al."Sphingomonas prati sp nov., isolated from alpine meadow soil".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 66(2016).