气候变化领域集成服务门户(CCPortal): Altitudinal Distribution of Ammonia-Oxidizing Archaea and Bacteria in Alpine Grassland Soils Along the South-Facing Slope of Nyqentangula Mountains, Central Tibetan Plateau

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DOI	10.1080/01490451.2014.925013
	Altitudinal Distribution of Ammonia-Oxidizing Archaea and Bacteria in Alpine Grassland Soils Along the South-Facing Slope of Nyqentangula Mountains, Central Tibetan Plateau
	Yuan, Yanli; Si, Guicai; Li, Wei; Zhang, Gengxin
通讯作者	Zhang, GX (通讯作者)
发表日期	2015
ISSN	0149-0451
EISSN	1521-0529
起始页码	77
结束页码	88
卷号	32 期号:1
英文摘要	Nitrogen is a major limiting nutrient for the net primary production of terrestrial ecosystems, especially on sentinel alpine ecosystem. Ammonia oxidation is the first and rate-limiting step on nitrification process and is thus crucial to nitrogen cycle. To decipher climatic influence on ammonia oxidizers, their communities were characterized by qPCR and clone sequencing by targeting amoA genes (encoding the alpha subunit of ammonia mono-oxygenase) in soils from 7 sites over an 800m elevation transect (4400-5200m a.s.l.), based on space-to-time substitution strategy, on a steppe-meadow ecosystem located on the central Tibetan Plateau (TP). Archaeal amoA abundance outnumbered bacterial amoA abundance at lower altitude (<4800m a.s.l.), but bacterial amoA abundance was greater in surface soils at higher altitude (>= 4800m a.s.l.). Archaeal amoA abundance decreased with altitude in surface soil, while its abundance stayed relatively stable and was mostly greater than bacterial amoA abundance in subsurface soils. Conversely, bacterial amoA abundance gradually increased with altitude at all three soil depths. Statistical analysis indicated that altitude-dependent factors, in particular pH and precipitation, had a profound effect on the abundance and community of ammonia-oxidizing bacteria, but only on the community composition of ammonia-oxidizing archaea along the altitudinal gradient. These findings imply that the shifts in the relative abundance and/or community structure of ammonia-oxidizing bacteria and archaea may result from the precipitation variation along the altitudinal gradient. Thus, we speculate that altitude-related factors (mainly precipitation variation combing changed pH), would play a vital role in affecting nitrification process on this alpine grassland ecosystem located at semi-arid area on TP.
关键词	COMMUNITY STRUCTURE AGRICULTURAL SOILS ACIDIC SOILS ABUNDANCE NITRIFICATION DIVERSITY NITROGEN CHINA FERTILIZATION PRODUCTIVITY
英文关键词	semi-arid grassland; amoA gene; ammonia oxidizer; Tibetan Plateau
语种	英语
WOS研究方向	Environmental Sciences & Ecology ; Geology
WOS类目	Environmental Sciences ; Geosciences, Multidisciplinary
WOS记录号	WOS:000344595900009
来源期刊	GEOMICROBIOLOGY JOURNAL
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/258497
推荐引用方式 GB/T 7714	Yuan, Yanli,Si, Guicai,Li, Wei,et al. Altitudinal Distribution of Ammonia-Oxidizing Archaea and Bacteria in Alpine Grassland Soils Along the South-Facing Slope of Nyqentangula Mountains, Central Tibetan Plateau[J]. 中国科学院青藏高原研究所,2015,32(1).
APA	Yuan, Yanli,Si, Guicai,Li, Wei,&Zhang, Gengxin.(2015).Altitudinal Distribution of Ammonia-Oxidizing Archaea and Bacteria in Alpine Grassland Soils Along the South-Facing Slope of Nyqentangula Mountains, Central Tibetan Plateau.GEOMICROBIOLOGY JOURNAL,32(1).
MLA	Yuan, Yanli,et al."Altitudinal Distribution of Ammonia-Oxidizing Archaea and Bacteria in Alpine Grassland Soils Along the South-Facing Slope of Nyqentangula Mountains, Central Tibetan Plateau".GEOMICROBIOLOGY JOURNAL 32.1(2015).