气候变化领域集成服务门户(CCPortal): Diversity and distribution of autotrophic microbial community along environmental gradients in grassland soils on the Tibetan Plateau

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DOI	10.1007/s00253-015-6723-x
	Diversity and distribution of autotrophic microbial community along environmental gradients in grassland soils on the Tibetan Plateau
	Guo, Guangxia; Kong, Weidong; Liu, Jinbo; Zhao, Jingxue; Du, Haodong; Zhang, Xianzhou; Xia, Pinhua
通讯作者	Guo, GX (通讯作者)
发表日期	2015
ISSN	0175-7598
EISSN	1432-0614
起始页码	8765
结束页码	8776
卷号	99 期号:20
英文摘要	Soil microbial autotrophs play a significant role in CO2 fixation in terrestrial ecosystem, particularly in vegetation-constrained ecosystems with environmental stresses, such as the Tibetan Plateau characterized by low temperature and high UV. However, soil microbial autotrophic communities and their driving factors remain less appreciated. We investigated the structure and shift of microbial autotrophic communities and their driving factors along an elevation gradient (4400-5100 m above sea level) in alpine grassland soils on the Tibetan Plateau. The autotrophic microbial communities were characterized by quantitative PCR, terminal restriction fragment length polymorphism (T-RFLP), and cloning/sequencing of cbbL genes, encoding the large subunit for the CO2 fixation protein ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO). High cbbL gene abundance and high RubisCO enzyme activity were observed and both significantly increased with increasing elevations. Path analysis identified that soil RubisCO enzyme causally originated from microbial autotrophs, and its activity was indirectly driven by soil water content, temperature, and NH4 (+) content. Soil autotrophic microbial community structure dramatically shifted along the elevation and was jointly driven by soil temperature, water content, nutrients, and plant types. The autotrophic microbial communities were dominated by bacterial autotrophs, which were affiliated with Rhizobiales, Burkholderiales, and Actinomycetales. These autotrophs have been well documented to degrade organic matters; thus, metabolic versatility could be a key strategy for microbial autotrophs to survive in the harsh environments. Our results demonstrated high abundance of microbial autotrophs and high CO2 fixation potential in alpine grassland soils and provided a novel model to identify dominant drivers of soil microbial communities and their ecological functions.
关键词	LARGE-SUBUNIT GENES ALTITUDINAL GRADIENT BACTERIAL COMMUNITY ELEVATION GRADIENT ALPINE GRASSLANDS SPECIES RICHNESS CARBON-DIOXIDE CO2 FIXATION FORM-II ASSIMILATION
英文关键词	RubisCO; CO2 fixation; Tibetan Plateau; Grassland; Elevation
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000361493200038
来源期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/258476
推荐引用方式 GB/T 7714	Guo, Guangxia,Kong, Weidong,Liu, Jinbo,et al. Diversity and distribution of autotrophic microbial community along environmental gradients in grassland soils on the Tibetan Plateau[J]. 中国科学院青藏高原研究所,2015,99(20).
APA	Guo, Guangxia.,Kong, Weidong.,Liu, Jinbo.,Zhao, Jingxue.,Du, Haodong.,...&Xia, Pinhua.(2015).Diversity and distribution of autotrophic microbial community along environmental gradients in grassland soils on the Tibetan Plateau.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,99(20).
MLA	Guo, Guangxia,et al."Diversity and distribution of autotrophic microbial community along environmental gradients in grassland soils on the Tibetan Plateau".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 99.20(2015).