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DOI	10.1016/j.ijbiomac.2014.11.045
	In vivo and in vitro observations of polyhydroxybutyrate granules formed by Dinoroseobacter sp JL 1447
	Xiao, Na; Jiao, Nianzhi; Liu, Yongqin
通讯作者	Jiao, NZ (通讯作者)
发表日期	2015
ISSN	0141-8130
EISSN	1879-0003
起始页码	467
结束页码	475
卷号	74
英文摘要	Polyhydroxybutyrate (PHB) granules formed by a marine aerobic anoxygenic phototrophic bacterial strain Dinoroseobacter sp. JL 1447 were detected using transmission electron microscopy and atomic force microscopy. When Dinoroseobacter sp. JL 1447 was inoculated into a medium with glucose as the sole carbon source, the formation of PHB granules occurred and accumulated with incubation time, reaching their maximum in the stationary phase cultures. PHB granules, formed in the cytoplasm at the cell poles or future cell poles, were remobilized and used by the cells in late stationary complex cultures. When PHB granules formed, cell length elongated from 0.5 to 1.5 mu m and spherical protrusions appeared on the cell surface. The French press method was used to break the cells and isolate the PHB granules. The freshly prepared and intact PHB granules were spherical with a soft, smooth outer envelope without visible substructures. Upon treating PHB granules with sodium dodecyl sulfate, the envelope was destroyed and nearly parted from the granules, and uniform, spherical structures with a central pore appeared on the granule surface. (C) 2015 Published by Elsevier B.V.
关键词	ATOMIC-FORCE MICROSCOPY ANOXYGENIC PHOTOTROPHIC BACTERIA POLYHYDROXYALKANOATE INCLUSIONS RALSTONIA-EUTROPHA PSEUDOMONAS-OLEOVORANS ELECTRON-MICROSCOPY PHB GRANULES H16 LOCALIZATION PROTEIN
英文关键词	Polyhydroxybutyrate granules; Atomic force microscopy (AFM); Transmission electron microscopy (TEM); Morphology
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:000351248600061
来源期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/258435
推荐引用方式 GB/T 7714	Xiao, Na,Jiao, Nianzhi,Liu, Yongqin. In vivo and in vitro observations of polyhydroxybutyrate granules formed by Dinoroseobacter sp JL 1447[J]. 中国科学院青藏高原研究所,2015,74.
APA	Xiao, Na,Jiao, Nianzhi,&Liu, Yongqin.(2015).In vivo and in vitro observations of polyhydroxybutyrate granules formed by Dinoroseobacter sp JL 1447.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,74.
MLA	Xiao, Na,et al."In vivo and in vitro observations of polyhydroxybutyrate granules formed by Dinoroseobacter sp JL 1447".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 74(2015).