气候变化领域集成服务门户(CCPortal): The microbial gene diversity along an elevation gradient of the Tibetan grassland

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DOI	10.1038/ismej.2013.146
	The microbial gene diversity along an elevation gradient of the Tibetan grassland
	Yang, Yunfeng; Gao, Ying; Wang, Shiping; Xu, Depeng; Yu, Hao; Wu, Linwei; Lin, Qiaoyan; Hu, Yigang; Li, Xiangzhen; He, Zhili; Deng, Ye; Zhou, Jizhong
通讯作者	Yang, YF (通讯作者)
发表日期	2014
ISSN	1751-7362
EISSN	1751-7370
起始页码	430
结束页码	440
卷号	8 期号:2
英文摘要	Tibet is one of the most threatened regions by climate warming, thus understanding how its microbial communities function may be of high importance for predicting microbial responses to climate changes. Here, we report a study to profile soil microbial structural genes, which infers functional roles of microbial communities, along four sites/elevations of a Tibetan mountainous grassland, aiming to explore the potential microbial responses to climate changes via a strategy of space-for-time substitution. Using a microarray-based metagenomics tool named GeoChip 4.0, we showed that microbial communities were distinct for most but not all of the sites. Substantial variations were apparent in stress, N and C-cycling genes, but they were in line with the functional roles of these genes. Cold shock genes were more abundant at higher elevations. Also, gdh converting ammonium into urea was more abundant at higher elevations, whereas ureC converting urea into ammonium was less abundant, which was consistent with soil ammonium contents. Significant correlations were observed between N-cycling genes (ureC, gdh and amoA) and nitrous oxide flux, suggesting that they contributed to community metabolism. Lastly, we found by Canonical correspondence analysis, Mantel tests and the similarity tests that soil pH, temperature, NH4+-N and vegetation diversity accounted for the majority (81.4%) of microbial community variations, suggesting that these four attributes were major factors affecting soil microbial communities. On the basis of these observations, we predict that climate changes in the Tibetan grasslands are very likely to change soil microbial community functional structure, with particular impacts on microbial N-cycling genes and consequently microbe-mediated soil N dynamics.
关键词	ECOSYSTEM CO2 EXCHANGE GEOCHIP-BASED ANALYSIS ALPINE MEADOW BACTERIAL COMMUNITIES SOIL-MOISTURE PLATEAU SEA PATTERNS NITROGEN DESERT
英文关键词	gene diversity; soil microbial community; community metabolism; alpine grassland; elevation gradient
语种	英语
WOS研究方向	Environmental Sciences & Ecology ; Microbiology
WOS类目	Ecology ; Microbiology
WOS记录号	WOS:000330386500016
来源期刊	ISME JOURNAL
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/258397
推荐引用方式 GB/T 7714	Yang, Yunfeng,Gao, Ying,Wang, Shiping,et al. The microbial gene diversity along an elevation gradient of the Tibetan grassland[J]. 中国科学院青藏高原研究所,2014,8(2).
APA	Yang, Yunfeng.,Gao, Ying.,Wang, Shiping.,Xu, Depeng.,Yu, Hao.,...&Zhou, Jizhong.(2014).The microbial gene diversity along an elevation gradient of the Tibetan grassland.ISME JOURNAL,8(2).
MLA	Yang, Yunfeng,et al."The microbial gene diversity along an elevation gradient of the Tibetan grassland".ISME JOURNAL 8.2(2014).