气候变化领域集成服务门户(CCPortal): Proteasome-Mediated Degradation of FRIGIDA Modulates Flowering Time in Arabidopsis during Vernalization

CCPortal

DOI	10.1105/tpc.114.132738
	Proteasome-Mediated Degradation of FRIGIDA Modulates Flowering Time in Arabidopsis during Vernalization
	Hu, Xiangyang; Kong, Xiangxiang; Wang, Chuntao; Ma, Lan; Zhao, Jinjie; Wei, Jingjing; Zhang, Xiaoming; Loake, Gary J.; Zhang, Ticao; Huang, Jinling; Yang, Yongping
通讯作者	Hu, XY (通讯作者)
发表日期	2014
ISSN	1040-4651
EISSN	1532-298X
起始页码	4763
结束页码	4781
卷号	26 期号:12
英文摘要	Winter-annual accessions of Arabidopsis thaliana require either exposure to cold stress or vernalization to initiate flowering via FRIGIDA (FRI). FRI acts as a scaffold protein to recruit several chromatin modifiers that epigenetically modify flowering genes. Here, we report that proteasome-mediated FRI degradation regulates flowering during vernalization in Arabidopsis. Our genetic and biochemical experiments demonstrate that FRI directly interacts with the BTB (Bric-a-Brac/Tramtrack/Broad Complex) proteins LIGHT-RESPONSE BTB1 (LRB1) and LRB2 as well as the CULLIN3A (CUL3A) ubiquitin-E3 ligase in vitro and in vivo, leading to proteasomal degradation of FRI during vernalization. The degradation of FRI is accompanied by an increase in the levels of the long noncoding RNA ColdAIR, which reduces the level of histone H3Lys4 trimethylation (H3K4me3) in FLOWERING LOCUS C chromatin to promote flowering. Furthermore, we found that the cold-induced WRKY34 transcription factor binds to the W-box in the promoter region of CUL3A to modulate CUL3A expression. Deficiency of WRKY34 suppressed CUL3A transcription to enhance FRI protein stability and led to late flowering after vernalization. Conversely, overexpression of WRK34 promoted FRI degradation and early flowering through inducing CUL3A accumulation. Together, these data suggest that WRKY34-induced and CUL3A-dependent proteolysis of FRI modulate flowering in response to vernalization.
关键词	WINTER-ANNUAL HABIT CHROMATIN MODIFICATION HISTONE METHYLATION NATURAL VARIATION NONCODING RNA CAJAL BODIES E3 LIGASE TRANSCRIPTION PROTEINS THALIANA
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS记录号	WOS:000348646000018
来源期刊	PLANT CELL
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/258386
推荐引用方式 GB/T 7714	Hu, Xiangyang,Kong, Xiangxiang,Wang, Chuntao,et al. Proteasome-Mediated Degradation of FRIGIDA Modulates Flowering Time in Arabidopsis during Vernalization[J]. 中国科学院青藏高原研究所,2014,26(12).
APA	Hu, Xiangyang.,Kong, Xiangxiang.,Wang, Chuntao.,Ma, Lan.,Zhao, Jinjie.,...&Yang, Yongping.(2014).Proteasome-Mediated Degradation of FRIGIDA Modulates Flowering Time in Arabidopsis during Vernalization.PLANT CELL,26(12).
MLA	Hu, Xiangyang,et al."Proteasome-Mediated Degradation of FRIGIDA Modulates Flowering Time in Arabidopsis during Vernalization".PLANT CELL 26.12(2014).