气候变化领域集成服务门户(CCPortal): Molecular cloning and characterization of a novel SK3-type dehydrin gene from Stipa purpurea

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DOI	10.1016/j.bbrc.2014.04.075
	Molecular cloning and characterization of a novel SK3-type dehydrin gene from Stipa purpurea
	Yang, Yunqiang; Sun, Xudong; Yang, Shihai; Li, Xiong; Yang, Yongping
通讯作者	Yang, YP (通讯作者)
发表日期	2014
ISSN	0006-291X
EISSN	1090-2104
起始页码	145
结束页码	150
卷号	448 期号:2
英文摘要	Stipa purpurea, an endemic forage of the grass family in the Tibetan Plateau, is highly resistant to drought. Dehydrins (DHNs) are stress proteins involved in plant protective reactions against environmental stress. In this study, the full-length DHN open reading frame (ORF) cloned from S. purpurea, named SpDHN1, was 816 nucleotides length and encoded a protein of 271 amino acids. Phylogenetic and sequence characterization analysis revealed that the DHN gene was an SK3-type DHNs. Subcellular localization analysis indicated that SpDHN1 was localized in the cytoplasm and the plasma membrane. SpDHN1 function analysis provided new evidence to support the antioxidation of SpDHN1 in plant responses drought stress. Ectopic expression of SpDHN1 in Arabidopsis thaliana plants showed more resistance to drought stress than the wild-type, indicating that SpDHN1 may be a potential candidate gene for genetic improvement of crops to improve stress tolerance. (C) 2014 Elsevier Inc. All rights reserved.
关键词	COLD-ACCLIMATION PLANT DEHYDRINS DROUGHT STRESS TOLERANCE FAMILY SALT EXPRESSION PATTERN
英文关键词	Stipa purpurea; Dehydrin; Cloning; Drought; Antioxidation
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000336887000005
来源期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/258286
推荐引用方式 GB/T 7714	Yang, Yunqiang,Sun, Xudong,Yang, Shihai,et al. Molecular cloning and characterization of a novel SK3-type dehydrin gene from Stipa purpurea[J]. 中国科学院青藏高原研究所,2014,448(2).
APA	Yang, Yunqiang,Sun, Xudong,Yang, Shihai,Li, Xiong,&Yang, Yongping.(2014).Molecular cloning and characterization of a novel SK3-type dehydrin gene from Stipa purpurea.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,448(2).
MLA	Yang, Yunqiang,et al."Molecular cloning and characterization of a novel SK3-type dehydrin gene from Stipa purpurea".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 448.2(2014).