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DOI	10.1099/ijs.0.041285-0
	Brockia lithotrophica gen. nov., sp nov., an anaerobic thermophilic bacterium from a terrestrial hot spring
	Perevalova, Anna A.; Kublanov, Ilya V.; Baslerov, R. V.; Zhang, Gengxin; Bonch-Osmolovskaya, Elizaveta A.
通讯作者	Perevalova, AA (通讯作者)
发表日期	2013
ISSN	1466-5026
EISSN	1466-5034
起始页码	479
结束页码	483
卷号	63
英文摘要	A novel thermophilic bacterium, strain Kam1851(T), was isolated from a terrestrial hot spring of the Uzon Caldera, Kamchatka Peninsula, Russia. Cells of strain Kam1851(T) were spore-forming rods with a Gram-positive type of cell wall. Growth was observed between 46 and 78 degrees C, and pH 5.5-8.5. The optimal growth (doubling time, 6.0 h) was at 60-65 degrees C and pH 6.5. The isolate was an obligate anaerobe growing in pre-reduced medium only. It grew on mineral medium with molecular hydrogen or formate as electron donors, and elemental sulfur, thiosulfate or polysulfide as electron acceptors. The main cellular fatty acids were C-16:0 (34.2 %), iso-C-16:0 (18 %), C-18:0 (12.8 %) and iso-C-17:0 (11.1 %). The G+C content of the genomic DNA of strain Kam1851(T) was 63 mol%. 16S rRNA gene sequence analysis showed that strain Kam1851(T) belonged to the order Thermoanaerobacterales, but it was not closely related to representatives of any genera with validly published names. The most closely related strains, which had no more than 89.2% sequence similarity, were members of the genera Ammonifex and Caldanaerobacter. On the basis of its phylogenetic position and novel phenotypic features, isolate Kam1851(T) is proposed to represent a novel species in a new genus, Brockia lithotrophica gen. nov., sp. nov.; the type strain of Brockia lithotrophica is Kam1851(T) (=DSM 22653(T)=VKM B-2685(T)).
关键词	EMENDED DESCRIPTION UZON CALDERA PHYLOGENIES ACID
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000317170400013
来源期刊	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/258128
推荐引用方式 GB/T 7714	Perevalova, Anna A.,Kublanov, Ilya V.,Baslerov, R. V.,et al. Brockia lithotrophica gen. nov., sp nov., an anaerobic thermophilic bacterium from a terrestrial hot spring[J]. 中国科学院青藏高原研究所,2013,63.
APA	Perevalova, Anna A.,Kublanov, Ilya V.,Baslerov, R. V.,Zhang, Gengxin,&Bonch-Osmolovskaya, Elizaveta A..(2013).Brockia lithotrophica gen. nov., sp nov., an anaerobic thermophilic bacterium from a terrestrial hot spring.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,63.
MLA	Perevalova, Anna A.,et al."Brockia lithotrophica gen. nov., sp nov., an anaerobic thermophilic bacterium from a terrestrial hot spring".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 63(2013).