气候变化领域集成服务门户(CCPortal): Methanotrophic community structure and activity under warming and grazing of alpine meadow on the Tibetan Plateau

CCPortal

DOI	10.1007/s00253-011-3535-5
	Methanotrophic community structure and activity under warming and grazing of alpine meadow on the Tibetan Plateau
	Zheng, Yong; Yang, Wei; Sun, Xiang; Wang, Shi-Ping; Rui, Yi-Chao; Luo, Cai-Yun; Guo, Liang-Dong
通讯作者	Guo, LD (通讯作者)
发表日期	2012
ISSN	0175-7598
起始页码	2193
结束页码	2203
卷号	93 期号:5
英文摘要	Knowledge about methanotrophs and their activities is important to understand the microbial mediation of the greenhouse gas CH4 under climate change and human activities in terrestrial ecosystems. The effects of simulated warming and sheep grazing on methanotrophic abundance, community composition, and activity were studied in an alpine meadow soil on the Tibetan Plateau. There was high abundance of methanotrophs (1.2-3.4 x 10(8) pmoA gene copies per gram of dry weight soil) assessed by real-time PCR, and warming significantly increased the abundance regardless of grazing. A total of 64 methanotrophic operational taxonomic units (OTUs) were obtained from 1,439 clone sequences, of these OTUs; 63 OTUs (98.4%) belonged to type I methanotrophs, and only one OTU was Methylocystis of type II methanotrophs. The methanotroph community composition and diversity were not apparently affected by the treatments. Warming and grazing significantly enhanced the potential CH4 oxidation activity. There were significantly negative correlations between methanotrophic abundance and soil moisture and between methanotrophic abundance and NH4-N content. The study suggests that type I methanotrophs, as the dominance, may play a key role in CH4 oxidation, and the alpine meadow has great potential to consume more CH4 under future warmer and grazing conditions on the Tibetan Plateau.
关键词	METHANE-OXIDIZING BACTERIA LANDFILL COVER SOIL 16S RIBOSOMAL-RNA MICROBIAL OXIDATION PLANT-COMMUNITIES GRASSLAND SOIL CLIMATE-CHANGE FOREST SOIL ECOSYSTEM TEMPERATURE
英文关键词	Clone library; Grazing; Methanotrophs; pmoA; Tibetan Plateau; Real-time
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000300657900036
来源期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/257999
推荐引用方式 GB/T 7714	Zheng, Yong,Yang, Wei,Sun, Xiang,et al. Methanotrophic community structure and activity under warming and grazing of alpine meadow on the Tibetan Plateau[J]. 中国科学院青藏高原研究所,2012,93(5).
APA	Zheng, Yong.,Yang, Wei.,Sun, Xiang.,Wang, Shi-Ping.,Rui, Yi-Chao.,...&Guo, Liang-Dong.(2012).Methanotrophic community structure and activity under warming and grazing of alpine meadow on the Tibetan Plateau.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,93(5).
MLA	Zheng, Yong,et al."Methanotrophic community structure and activity under warming and grazing of alpine meadow on the Tibetan Plateau".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 93.5(2012).