气候变化领域集成服务门户(CCPortal): A study on the vertical profile of bacterial DNA structure in the Puruogangri (Tibetan Plateau) ice core using denaturing gradient gel electrophoresis

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DOI	10.3189/172756406781811934
	A study on the vertical profile of bacterial DNA structure in the Puruogangri (Tibetan Plateau) ice core using denaturing gradient gel electrophoresis
	Zhang, Xinfang; Ya, Tandong; An, Lizhe; Tian, Lide; Xu, Shijian
发表日期	2006
ISSN	0260-3055
起始页码	160
结束页码	+
卷号	43
英文摘要	The bacterial DNA structures at different depths in the Puruogangri (Tibetan Plateau) ice core (83.45 m) were investigated by the denaturing gradient gel electrophoresis (DGGE) DNA fingerprinting technique. DGGE profiles indicated that the bacterial species diversity in glacial ice is high, and indigenous species represented by common hands in all samples may grow on the glacial surface. Bacterial diversity, as estimated by Shannon indices (mean 2.91; SD 0.25; n = 13), was comparable to that Of soil habitats and had a positive correlation with Ca2+ concentration (R = 0.71; P < 0.01), a good proxy of dust. This suggested that the soil ecosystem was the main source of bacteria in this glacier. The low similarity indices (0-43%) were found between the ice-core samples, which corresponded to the episodic deposition under defined climatic conditions and low activity of microorganisms in glacial ice. The profiles of bacterial species composition in glacial ice may be a bio-indicator of climatic changes or dating.
关键词	16S RIBOSOMAL-RNA ANCIENT GLACIAL ICE MICROBIAL COMMUNITIES DEFINED POPULATIONS LAKE VOSTOK ANTARCTICA DIVERSITY MICROORGANISMS RECOVERY RDNA
语种	英语
WOS研究方向	Geology
WOS类目	Geosciences, Multidisciplinary
WOS记录号	WOS:000245520800023
来源期刊	ANNALS OF GLACIOLOGY, VOL 43, 2006
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/257432
推荐引用方式 GB/T 7714	Zhang, Xinfang,Ya, Tandong,An, Lizhe,et al. A study on the vertical profile of bacterial DNA structure in the Puruogangri (Tibetan Plateau) ice core using denaturing gradient gel electrophoresis[J]. 中国科学院青藏高原研究所,2006,43.
APA	Zhang, Xinfang,Ya, Tandong,An, Lizhe,Tian, Lide,&Xu, Shijian.(2006).A study on the vertical profile of bacterial DNA structure in the Puruogangri (Tibetan Plateau) ice core using denaturing gradient gel electrophoresis.ANNALS OF GLACIOLOGY, VOL 43, 2006,43.
MLA	Zhang, Xinfang,et al."A study on the vertical profile of bacterial DNA structure in the Puruogangri (Tibetan Plateau) ice core using denaturing gradient gel electrophoresis".ANNALS OF GLACIOLOGY, VOL 43, 2006 43(2006).