气候变化领域集成服务门户(CCPortal): Bacterial diversity in Malan ice core from the Tibetan Plateau

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DOI	10.1007/BF02931042
	Bacterial diversity in Malan ice core from the Tibetan Plateau
	Xiang, SR; Yao, TD; An, LZ; Xu, BQ; Li, Z; Wu, GJ; Wang, YQ; Ma, S; Chen, XR
发表日期	2004
ISSN	0015-5632
EISSN	1874-9356
起始页码	269
结束页码	275
卷号	49 期号:3
英文摘要	Three ice core samples were collected from the Malan ice core drilled from the Tibetan Plateau, and three 16S rDNA clone libraries by direct amplification from the ice-melted water were established. Ninety-four clones containing bacterial 16S rDNA inserts were selected. According to restriction fragment-length polymorphism analysis, 11 clones were unique in the library from which they were obtained and used for partial sequence and phylogenetic analysis, and compared with 8 reported sequences from the same ice core at depth 70 m. Differences among the samples were apparent in clone libraries. The phylotypes were dominated by the Proteobacteria group, Acinetobacter sp. and Cytophaga Flavobacterium Bacteroides (CFB) group. They accounted for 92.5% (Proteobacteria), 100% (Acinetobacter sp.), 34.4% (CFB) and 100% (beta-Proteobacteria) in the clone libraries from the samples at ice depths 35, 64, 70, and 82 m, respectively. The Acinetobacter sp. was only found in the deposition at ice depth 82 m and closely clustered with gamma-Proteobateria. Two members (Malan A-21 and 101) of alpha-Proteobacteria from the sample of 35 m and two (Malan B-26 and 48) of beta-Proteobacteria of 64 m were loosely clustered (<95% similarity) with known bacteria, represented new genera in ice bacteria.
关键词	ANTARCTIC SEA-ICE VIABLE BACTERIA LAKES
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000221874600005
来源期刊	FOLIA MICROBIOLOGICA
来源机构	中国科学院青藏高原研究所
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/257358
推荐引用方式 GB/T 7714	Xiang, SR,Yao, TD,An, LZ,et al. Bacterial diversity in Malan ice core from the Tibetan Plateau[J]. 中国科学院青藏高原研究所,2004,49(3).
APA	Xiang, SR.,Yao, TD.,An, LZ.,Xu, BQ.,Li, Z.,...&Chen, XR.(2004).Bacterial diversity in Malan ice core from the Tibetan Plateau.FOLIA MICROBIOLOGICA,49(3).
MLA	Xiang, SR,et al."Bacterial diversity in Malan ice core from the Tibetan Plateau".FOLIA MICROBIOLOGICA 49.3(2004).